Detection of circulating tumor cells in patients

Detection of circulating tumor cells in patients with breast cancer using the conditionally reprogrammed cell culture method and reverse transcription-PCR of hTERT and MAGE A1-6

The present study aimed to verify the efficacy of the conditionally reprogrammed cell (CRC) culture method for the detection of circulating tumor cells (CTCs) in breast cancer. CTCs were isolated from the peripheral blood of patients with breast cancer, and culture of the collected CTCs was performed according to the conditional reprogramming protocol. Total RNA was extracted from cultured CTCs, and the hTERT and MAGE A1-6 genes were amplified using reverse transcription-PCR (RT-PCR). In addition, RNA extraction from another blood sample was performed and the expression of the two genes was analyzed by RT-PCR only. Following CRC culture, grown CTCs were observed in 7 samples (23.3%). The CTC detection rates by RT-PCR for the hTERT and MAGE A1-6 genes in CTCs grown using the CRC culture method were 26.7 and 10.0%, respectively. The positive expression rates for the hTERT and MAGE genes in CTCs assessed by RT-PCR only were 44.1 and 23.5%, respectively. When combining the positive expression rates of RT-PCR only and CRC culture for the hTERT and MAGE A1-6 genes, CTC detection rates increased to 53.3 and 23.3%, respectively. Additionally, when combining the positive expression rates of the two genes by either method, the CTC detection rate was the highest value observed. In conclusion, the present study revealed the potential of CRC culture in the detection of CTCs in breast cancer. Furthermore, a combination of CRC culture and RT-PCR for the hTERT and MAGE A1-6 genes is useful in enhancing the detection rate of CTCs in the blood.

GPR81 Antibody

ABD2766 100 ug
EUR 438

GPR81 Antibody

44942-100ul 100ul
EUR 252

GPR81 Antibody

44942-50ul 50ul
EUR 187

GPR81 antibody

20R-GR037 50 ug
EUR 679
Description: Rabbit polyclonal GPR81 antibody

GPR81 antibody

20R-GR038 50 ug
EUR 679
Description: Rabbit polyclonal GPR81 antibody

GPR81 antibody

70R-50946 100 ul
EUR 244
Description: Purified Polyclonal GPR81 antibody

Rabbit Polyclonal antibody Anti-CRBN

Anti-CRBN 50 µg
EUR 349

Polyclonal Goat Anti-GPR81 / FKSG80 Antibody

APR16285G 0.1 mg
EUR 484
Description: A polyclonal antibody raised in Goat that recognizes and binds to Human Goat Anti-GPR81 / FKSG80 . This antibody is tested and proven to work in the following applications:

GPR81 Conjugated Antibody

C44942 100ul
EUR 397

GPR81 Blocking Peptide

DF2766-BP 1mg
EUR 195

GPR81 Blocking Peptide

20-abx063821
  • EUR 272.00
  • EUR 411.00
  • 1 mg
  • 5 mg
  • Shipped within 5-10 working days.

pDNR-Dual-GPR81

PVT17473 2 ug
EUR 231

Polyclonal GPR81 Antibody (C-term)

APR16611G 0.1ml
EUR 484
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human GPR81 (C-term). This antibody is tested and proven to work in the following applications:

GPR81 ELISA KIT|Human

EF009971 96 Tests
EUR 689

Polyclonal Goat anti-GST α-form

GST-ANTI-1 50 uL
EUR 280

Polyclonal Goat anti-GST μ-form

GST-ANTI-2 50 uL
EUR 280

Polyclonal Goat anti-GST p-form

GST-ANTI-3 50 uL
EUR 280

Polyclonal FKSG80 / GPR81 Antibody (C-Terminus)

APR15995G 0.05mg
EUR 484
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human FKSG80 / GPR81 (C-Terminus). This antibody is tested and proven to work in the following applications:

Polyclonal FKSG80 / GPR81 Antibody (Extracellular Domain)

APR15996G 0.05mg
EUR 484
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human FKSG80 / GPR81 (Extracellular Domain). This antibody is tested and proven to work in the following applications:

Polyclonal FKSG80 / GPR81 Antibody (N-Terminus)

APR15997G 0.05mg
EUR 484
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human FKSG80 / GPR81 (N-Terminus). This antibody is tested and proven to work in the following applications:

Gpr81 ORF Vector (Mouse) (pORF)

ORF046573 1.0 ug DNA
EUR 506

Gpr81 ORF Vector (Rat) (pORF)

ORF067824 1.0 ug DNA
EUR 506

G Protein-Coupled Receptor 81 (GPR81) Antibody

20-abx008192
  • EUR 300.00
  • EUR 439.00
  • EUR 189.00
  • 100 ul
  • 200 ul
  • 30 ul
  • Shipped within 5-10 working days.

G Protein-Coupled Receptor 81 (GPR81) Antibody

abx028676-400ul 400 ul
EUR 523
  • Shipped within 5-10 working days.

G Protein-Coupled Receptor 81 (GPR81) Antibody

abx028676-80l 80 µl
EUR 286
  • Shipped within 5-10 working days.

G Protein-Coupled Receptor 81 (GPR81) Antibody

20-abx147554
  • EUR 425.00
  • EUR 342.00
  • 100 ug
  • 50 ug
  • Shipped within 5-10 working days.

G Protein-Coupled Receptor 81 (GPR81) Antibody

abx233610-100ug 100 ug
EUR 551
  • Shipped within 5-12 working days.

G Protein-Coupled Receptor 81 (GPR81) Antibody

abx430538-200ul 200 ul
EUR 384
  • Shipped within 1-3 working days.

Gpr81 sgRNA CRISPR Lentivector set (Mouse)

K3904701 3 x 1.0 ug
EUR 339

GPR81 sgRNA CRISPR Lentivector set (Human)

K0893201 3 x 1.0 ug
EUR 339

Gpr81 sgRNA CRISPR Lentivector set (Rat)

K6130201 3 x 1.0 ug
EUR 339

Gpr81 sgRNA CRISPR Lentivector (Mouse) (Target 1)

K3904702 1.0 ug DNA
EUR 154

Gpr81 sgRNA CRISPR Lentivector (Mouse) (Target 2)

K3904703 1.0 ug DNA
EUR 154

Gpr81 sgRNA CRISPR Lentivector (Mouse) (Target 3)

K3904704 1.0 ug DNA
EUR 154

GPR81 sgRNA CRISPR Lentivector (Human) (Target 1)

K0893202 1.0 ug DNA
EUR 154

GPR81 sgRNA CRISPR Lentivector (Human) (Target 2)

K0893203 1.0 ug DNA
EUR 154

GPR81 sgRNA CRISPR Lentivector (Human) (Target 3)

K0893204 1.0 ug DNA
EUR 154

Gpr81 sgRNA CRISPR Lentivector (Rat) (Target 1)

K6130202 1.0 ug DNA
EUR 154

Gpr81 sgRNA CRISPR Lentivector (Rat) (Target 2)

K6130203 1.0 ug DNA
EUR 154

Gpr81 sgRNA CRISPR Lentivector (Rat) (Target 3)

K6130204 1.0 ug DNA
EUR 154

GPR81 Protein Vector (Mouse) (pPB-C-His)

PV186290 500 ng
EUR 603

GPR81 Protein Vector (Mouse) (pPB-N-His)

PV186291 500 ng
EUR 603

GPR81 Protein Vector (Mouse) (pPM-C-HA)

PV186292 500 ng
EUR 603

GPR81 Protein Vector (Mouse) (pPM-C-His)

PV186293 500 ng
EUR 603

GPR81 Protein Vector (Rat) (pPB-C-His)

PV271294 500 ng
EUR 603

GPR81 Protein Vector (Rat) (pPB-N-His)

PV271295 500 ng
EUR 603

GPR81 Protein Vector (Rat) (pPM-C-HA)

PV271296 500 ng
EUR 603

GPR81 Protein Vector (Rat) (pPM-C-His)

PV271297 500 ng
EUR 603

Gpr81 3'UTR Luciferase Stable Cell Line

TU205392 1.0 ml Ask for price

GPR81 3'UTR GFP Stable Cell Line

TU059183 1.0 ml
EUR 1521

Gpr81 3'UTR GFP Stable Cell Line

TU255392 1.0 ml Ask for price

GPR81 3'UTR Luciferase Stable Cell Line

TU009183 1.0 ml
EUR 1521

Gpr81 3'UTR Luciferase Stable Cell Line

TU109042 1.0 ml Ask for price

Gpr81 3'UTR GFP Stable Cell Line

TU159042 1.0 ml Ask for price

Gpr81 sgRNA CRISPR/Cas9 All-in-One Lentivector set (Mouse)

K3904705 3 x 1.0 ug
EUR 376

GPR81 sgRNA CRISPR/Cas9 All-in-One Lentivector set (Human)

K0893205 3 x 1.0 ug
EUR 376

Gpr81 sgRNA CRISPR/Cas9 All-in-One Lentivector set (Rat)

K6130205 3 x 1.0 ug
EUR 376

Gpr81 sgRNA CRISPR/Cas9 All-in-One Lentivector (Mouse) (Target 1)

K3904706 1.0 ug DNA
EUR 167

Gpr81 sgRNA CRISPR/Cas9 All-in-One Lentivector (Mouse) (Target 2)

K3904707 1.0 ug DNA
EUR 167

Gpr81 sgRNA CRISPR/Cas9 All-in-One Lentivector (Mouse) (Target 3)

K3904708 1.0 ug DNA
EUR 167

GPR81 sgRNA CRISPR/Cas9 All-in-One Lentivector (Human) (Target 1)

K0893206 1.0 ug DNA
EUR 167

GPR81 sgRNA CRISPR/Cas9 All-in-One Lentivector (Human) (Target 2)

K0893207 1.0 ug DNA
EUR 167

GPR81 sgRNA CRISPR/Cas9 All-in-One Lentivector (Human) (Target 3)

K0893208 1.0 ug DNA
EUR 167

Gpr81 sgRNA CRISPR/Cas9 All-in-One Lentivector (Rat) (Target 1)

K6130206 1.0 ug DNA
EUR 167

Gpr81 sgRNA CRISPR/Cas9 All-in-One Lentivector (Rat) (Target 2)

K6130207 1.0 ug DNA
EUR 167

Gpr81 sgRNA CRISPR/Cas9 All-in-One Lentivector (Rat) (Target 3)

K6130208 1.0 ug DNA
EUR 167

Anti-Anti-MARCH9 Antibody antibody

STJ112609 100 µl
EUR 277

Anti-Anti-SEPT11 Antibody antibody

STJ113941 100 µl
EUR 277

Anti-Anti-SEPT11 Antibody antibody

STJ114081 100 µl
EUR 277

Anti-Anti-SEPT5 Antibody antibody

STJ114819 100 µl
EUR 277
Description: This gene is a member of the septin gene family of nucleotide binding proteins, originally described in yeast as cell division cycle regulatory proteins. Septins are highly conserved in yeast, Drosophila, and mouse and appear to regulate cytoskeletal organization. Disruption of septin function disturbs cytokinesis and results in large multinucleate or polyploid cells. This gene is mapped to 22q11, the region frequently deleted in DiGeorge and velocardiofacial syndromes. A translocation involving the MLL gene and this gene has also been reported in patients with acute myeloid leukemia. Alternative splicing results in multiple transcript variants. The presence of a non-consensus polyA signal (AACAAT) in this gene also results in read-through transcription into the downstream neighboring gene (GP1BB; platelet glycoprotein Ib), whereby larger, non-coding transcripts are produced.

Anti-Anti-MARCH8 Antibody antibody

STJ114828 100 µl
EUR 277

Anti-Anti-SEPT7 Antibody antibody

STJ116214 100 µl
EUR 277
Description: This gene encodes a protein that is highly similar to the CDC10 protein of Saccharomyces cerevisiae. The protein also shares similarity with Diff 6 of Drosophila and with H5 of mouse. Each of these similar proteins, including the yeast CDC10, contains a GTP-binding motif. The yeast CDC10 protein is a structural component of the 10 nm filament which lies inside the cytoplasmic membrane and is essential for cytokinesis. This human protein functions in gliomagenesis and in the suppression of glioma cell growth, and it is required for the association of centromere-associated protein E with the kinetochore. Alternative splicing results in multiple transcript variants. Several related pseudogenes have been identified on chromosomes 5, 7, 9, 10, 11, 14, 17 and 19.

Anti-Anti-SEPT8 Antibody antibody

STJ117206 100 µl
EUR 277
Description: This gene is a member of the septin family of nucleotide binding proteins, originally described in yeast as cell division cycle regulatory proteins. Septins are highly conserved in yeast, Drosophila, and mouse, and appear to regulate cytoskeletal organization. Disruption of septin function disturbs cytokinesis and results in large multinucleate or polyploid cells. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Anti-Anti-SEPT12 Antibody antibody

STJ117759 100 µl
EUR 277
Description: This gene encodes a guanine-nucleotide binding protein and member of the septin family of cytoskeletal GTPases. Septins play important roles in cytokinesis, exocytosis, embryonic development, and membrane dynamics. Multiple transcript variants encoding different isoforms have been found for this gene.

Anti-Anti-MARCH6 Antibody antibody

STJ118549 100 µl
EUR 277

Anti-Anti-MARCH6 Antibody antibody

STJ118550 100 µl
EUR 277

Anti-Anti-MARCH7 Antibody antibody

STJ118752 100 µl
EUR 277

Anti-Anti-SEPT3 Antibody antibody

STJ118990 100 µl
EUR 277

Anti-Anti-SEPT1 antibody antibody

STJ119580 100 µl
EUR 277
Description: This gene is a member of the septin family of GTPases. Members of this family are required for cytokinesis and the maintenance of cellular morphology. This gene encodes a protein that can form homo- and heterooligomeric filaments, and may contribute to the formation of neurofibrillary tangles in Alzheimer's disease. Alternatively spliced transcript variants have been found but the full-length nature of these variants has not been determined. [provided by RefSeq, Dec 2012]

Anti-Anti-SEPT6 antibody antibody

STJ11100949 100 µl
EUR 277
Description: This gene is a member of the septin family of GTPases. Members of this family are required for cytokinesis. One version of pediatric acute myeloid leukemia is the result of a reciprocal translocation between chromosomes 11 and X, with the breakpoint associated with the genes encoding the mixed-lineage leukemia and septin 2 proteins. This gene encodes four transcript variants encoding three distinct isoforms. An additional transcript variant has been identified, but its biological validity has not been determined.

Anti-Anti-SEPT9 Antibody antibody

STJ111369 100 µl
EUR 277
Description: This gene is a member of the septin family involved in cytokinesis and cell cycle control. This gene is a candidate for the ovarian tumor suppressor gene. Mutations in this gene cause hereditary neuralgic amyotrophy, also known as neuritis with brachial predilection. A chromosomal translocation involving this gene on chromosome 17 and the MLL gene on chromosome 11 results in acute myelomonocytic leukemia. Multiple alternatively spliced transcript variants encoding different isoforms have been described.

Anti-Anti-SEPT11 Antibody antibody

STJ111530 100 µl
EUR 277

Anti-Anti-SEPT4 Antibody antibody

STJ112276 100 µl
EUR 277
Description: This gene is a member of the septin family of nucleotide binding proteins, originally described in yeast as cell division cycle regulatory proteins. Septins are highly conserved in yeast, Drosophila, and mouse, and appear to regulate cytoskeletal organization. Disruption of septin function disturbs cytokinesis and results in large multinucleate or polyploid cells. This gene is highly expressed in brain and heart. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. One of the isoforms (known as ARTS) is distinct; it is localized to the mitochondria, and has a role in apoptosis and cancer.

Anti-Anti-SEPT2 Antibody antibody

STJ25475 100 µl
EUR 277

Anti-Anti-SEPT5 Antibody antibody

STJ25477 100 µl
EUR 277
Description: This gene is a member of the septin gene family of nucleotide binding proteins, originally described in yeast as cell division cycle regulatory proteins. Septins are highly conserved in yeast, Drosophila, and mouse and appear to regulate cytoskeletal organization. Disruption of septin function disturbs cytokinesis and results in large multinucleate or polyploid cells. This gene is mapped to 22q11, the region frequently deleted in DiGeorge and velocardiofacial syndromes. A translocation involving the MLL gene and this gene has also been reported in patients with acute myeloid leukemia. Alternative splicing results in multiple transcript variants. The presence of a non-consensus polyA signal (AACAAT) in this gene also results in read-through transcription into the downstream neighboring gene (GP1BB; platelet glycoprotein Ib), whereby larger, non-coding transcripts are produced.

Anti-Anti-SEPT8 Antibody antibody

STJ25479 100 µl
EUR 277
Description: This gene is a member of the septin family of nucleotide binding proteins, originally described in yeast as cell division cycle regulatory proteins. Septins are highly conserved in yeast, Drosophila, and mouse, and appear to regulate cytoskeletal organization. Disruption of septin function disturbs cytokinesis and results in large multinucleate or polyploid cells. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Anti-Anti-SEPT2 Antibody antibody

STJ28365 100 µl
EUR 277

Anti-Anti-SEPT7 Antibody antibody

STJ28963 100 µl
EUR 277
Description: This gene encodes a protein that is highly similar to the CDC10 protein of Saccharomyces cerevisiae. The protein also shares similarity with Diff 6 of Drosophila and with H5 of mouse. Each of these similar proteins, including the yeast CDC10, contains a GTP-binding motif. The yeast CDC10 protein is a structural component of the 10 nm filament which lies inside the cytoplasmic membrane and is essential for cytokinesis. This human protein functions in gliomagenesis and in the suppression of glioma cell growth, and it is required for the association of centromere-associated protein E with the kinetochore. Alternative splicing results in multiple transcript variants. Several related pseudogenes have been identified on chromosomes 5, 7, 9, 10, 11, 14, 17 and 19.

Anti-Anti-DDB1 Antibody

A00333 100uL
EUR 455
Description: Rabbit Polyclonal DDB1 Antibody. Validated in IP and tested in Human, Mouse.

Human Anti-Anti-Sperm Antibody Antibody (Anti-AsAb) ELISA Kit

AEA465Hu-10x96wellstestplate 10x96-wells test plate
EUR 5647.8
  • The Intra-assay Precision is determined when 3 samples with low, middle and high level of Human Anti-Anti-Sperm Antibody Antibody (Anti-AsAb) were tested on 3 different plates, 8 replicates in each plate
  • CV(%) = SD/meanX100
  • Intra-Assay: CV<10%
  • Int
  • Show more
Description: This is Competitive Enzyme-linked immunosorbent assay for Antibody Detection.detection of Human Anti-Anti-Sperm Antibody Antibody (Anti-AsAb) in serum, plasma and other biological fluids.

Human Anti-Anti-Sperm Antibody Antibody (Anti-AsAb) ELISA Kit

AEA465Hu-1x48wellstestplate 1x48-wells test plate
EUR 552.76
  • The Intra-assay Precision is determined when 3 samples with low, middle and high level of Human Anti-Anti-Sperm Antibody Antibody (Anti-AsAb) were tested on 3 different plates, 8 replicates in each plate
  • CV(%) = SD/meanX100
  • Intra-Assay: CV<10%
  • Int
  • Show more
Description: This is Competitive Enzyme-linked immunosorbent assay for Antibody Detection.detection of Human Anti-Anti-Sperm Antibody Antibody (Anti-AsAb) in serum, plasma and other biological fluids.

Human Anti-Anti-Sperm Antibody Antibody (Anti-AsAb) ELISA Kit

AEA465Hu-1x96wellstestplate 1x96-wells test plate
EUR 746.8
  • The Intra-assay Precision is determined when 3 samples with low, middle and high level of Human Anti-Anti-Sperm Antibody Antibody (Anti-AsAb) were tested on 3 different plates, 8 replicates in each plate
  • CV(%) = SD/meanX100
  • Intra-Assay: CV<10%
  • Int
  • Show more
Description: This is Competitive Enzyme-linked immunosorbent assay for Antibody Detection.detection of Human Anti-Anti-Sperm Antibody Antibody (Anti-AsAb) in serum, plasma and other biological fluids.

Human Anti-Anti-Sperm Antibody Antibody (Anti-AsAb) ELISA Kit

AEA465Hu-5x96wellstestplate 5x96-wells test plate
EUR 3060.6
  • The Intra-assay Precision is determined when 3 samples with low, middle and high level of Human Anti-Anti-Sperm Antibody Antibody (Anti-AsAb) were tested on 3 different plates, 8 replicates in each plate
  • CV(%) = SD/meanX100
  • Intra-Assay: CV<10%
  • Int
  • Show more
Description: This is Competitive Enzyme-linked immunosorbent assay for Antibody Detection.detection of Human Anti-Anti-Sperm Antibody Antibody (Anti-AsAb) in serum, plasma and other biological fluids.

Human Anti-Anti-Sperm Antibody Antibody (Anti-AsAb) ELISA Kit

4-AEA465Hu
  • EUR 5698.00
  • EUR 3011.00
  • EUR 747.00
  • 10 plates of 96 wells
  • 5 plates of 96 wells
  • 1 plate of 96 wells
  • Known also as Anti-Sperm Antibody elisa. Alternative names of the recognized antigen: Anti-Spermatozoa Antibodies
  • Sperm Antibodies
  • Antisperm Antibodies
Description: Enzyme-linked immunosorbent assay based on the Competitive Inhibition method for detection of Human Anti-Anti-Sperm Antibody Antibody (Anti-AsAb) in samples from serum, plasma and other biological fluids with no significant corss-reactivity with analogues from other species.

ELISA kit for Human Anti-AsAb (Anti-Anti-Sperm Antibody Antibody)

ELK8071 1 plate of 96 wells
EUR 432
  • The microtiter plate provided in this kit has been pre-coated with an antigen. Standards or samples are then added to the appropriate microtiter plate wells with a Horseradish Peroxidase (HRP)-conjugated secondary antibody. After TMB substrate soluti
  • Show more
Description: A competitive Inhibition ELISA kit for detection of Anti-Anti-Sperm Antibody Antibody from Human in samples from blood, serum, plasma, cell culture fluid and other biological fluids.

Anti-H4K20me1 Antibody

CI1005 100ul
EUR 491
Description: Rabbit Polyclonal H4K20me1 Antibody. Validated in ChIP, ChIP-qPCR, WB and tested in Human.

Anti-H4K20me3 Antibody

CI1011 100ul
EUR 491
Description: Rabbit Polyclonal H4K20me3 Antibody. Validated in ChIP, ChIP-qPCR, WB and tested in Human.

Anti-TIP5 Antibody

CI1016 100ul
EUR 491
Description: Rabbit Polyclonal TIP5 Antibody. Validated in ChIP, ChIP-qPCR, WB and tested in Human, Mouse.


Incidence, clinical course and risk factor for recurrent PCR positivity in discharged COVID-19 patients in Guangzhou, China: A prospective cohort study

The phenomenon of COVID-19 patients tested positive for SARS-CoV-2 after discharge (redetectable as positive, RP) emerged globally. The data of incidence rate and risk factors for RP event and the clinical features of RP patients may provide recommendations for virus containment and cases management for COVID-19. We prospectively collected and analyzed the epidemiological, clinical and virological data from 285 adult patients with COVID-19 and acquired their definite clinical outcome (getting PCR positive or not during post-discharge surveillance). By March 10, 27 (9.5%) discharged patients had tested positive for SARS-CoV-2 in their nasopharyngeal swab after a median duration of 7·0 days (IQR 5·0-8·0). Compared to first admission, RP patients generally had milder clinical symptoms, lower viral load, shorter length of stay and improved pulmonary conditions at readmission (p<0.05). Elder RP patients (≥ 60 years old) were more likely to be symptomatic compared to younger patients (7/8, 87.5% vs. 3/19, 18.8%, p = 0.001) at readmission.

Age, sex, epidemiological history, clinical symptoms and underlying diseases were similar between RP and non-RP patients (p>0.05). A prolonged duration of viral shedding (>10 days) during the first hospitalization [adjusted odds ratio [aOR]: 5.82, 95% confidence interval [CI]: 2.50-13.57 for N gene; aOR: 9.64, 95% CI: 3.91-23.73 for ORF gene] and higher Ct value (ORF) in the third week of the first hospitalization (aOR: 0.69; 95% CI: 0.50-0.95) were associated with RP events. In conclusion, RP events occurred in nearly 10% of COVID-19 patients shortly after the negative tests, were not associated with worsening symptoms and unlikely reflect reinfection. Patients’ lack of efficiency in virus clearance was a risk factor for RP result. It is noteworthy that elder RP patients (≥ 60 years old) were more susceptible to clinical symptoms at readmission.

Ovine Paratuberculosis: Seroprevalence and comparison of fecal culture and direct fecal PCR assay

Johne’s disease is chronic, incurable disease, caused by Mycobacterium avium subsp. paratuberculosis (MAP). Most studies in Egypt focused on incidence of the disease in cattle but few studies were reported presence of antibodies against MAP in sheep. The present study determined the seroprevalence rate of MAP among sheep in four Governorates and assessed the associated risk factors to MAP-infection. The seroprevalence rate of MAP among sheep was non-significant varied between different Governorates, it was ranged between 3.75%-12.3%. The results revealed that the seroprevalence rate of the disease was significantly increased in diarrheic sheep (11 %, 95 %CI: 7.2-16.2) during spring (15 %, 95 %CI: 8.3-25) and summer (8%, 95 %CI: 4.13-13.8) seasons. Contrary, the age of sheep and contact with other ruminants like cattle or goats had non-significant effect of spreading of MAP-infection among sheep. The detection of MAP in feces of sheep was carried out using culture and PCR to determine the efficiency of both tests. The kappa test revealed good agreement between both tests for detection of MAP. The obtained finding confirms the presence of MAP among sheep in Egypt. So, the appropriate control measures should be taken to reduce spreading of the disease among sheep and reduce its economic losses.

GPR12 Antibody

44910-100ul 100ul
EUR 252

GPR12 Antibody

44910-50ul 50ul
EUR 187

GPR12 antibody

20R-GR041 50 ug
EUR 656
Description: Rabbit polyclonal GPR12 antibody

GPR12 Antibody

1-CSB-PA009742LA01HU
  • EUR 317.00
  • EUR 335.00
  • 100ug
  • 50ug
  • Form: Liquid
  • Buffer: Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 >95%, Protein G purified
Description: A polyclonal antibody against GPR12. Recognizes GPR12 from Human. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC, IF; Recommended dilution: WB:1:500-1:5000, IHC:1:20-1:200, IF:1:50-1:200

GPR12 Antibody

1-CSB-PA008905
  • EUR 222.00
  • EUR 195.00
  • 100ug
  • 50ug
  • Form: Liquid
  • Buffer: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Description: A polyclonal antibody against GPR12. Recognizes GPR12 from Human. This antibody is Unconjugated. Tested in the following application: WB, IF, ELISA;WB:1/500-1/2000.IF:1/200-1/1000.ELISA:1/5000

GPR12 antibody

70R-49804 100 ul
EUR 244
Description: Purified Polyclonal GPR12 antibody

GPR12 antibody

70R-30940 100 ug
EUR 327
Description: Rabbit polyclonal GPR12 antibody

GPR12

RA30050 50 ug
EUR 618

Gpr12/ Rat Gpr12 ELISA Kit

ELI-09764r 96 Tests
EUR 886

GPR12 Polyclonal Antibody

ABP54586-003ml 0.03ml
EUR 158
  • Immunogen information: Synthesized peptide derived from the N-terminal region of human GPR12 at AA rangle: 10-90
  • Applications tips:
Description: A polyclonal antibody for detection of GPR12 from Human. This GPR12 antibody is for WB, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the N-terminal region of human GPR12 at AA rangle: 10-90

GPR12 Polyclonal Antibody

ABP54586-01ml 0.1ml
EUR 289
  • Immunogen information: Synthesized peptide derived from the N-terminal region of human GPR12 at AA rangle: 10-90
  • Applications tips:
Description: A polyclonal antibody for detection of GPR12 from Human. This GPR12 antibody is for WB, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the N-terminal region of human GPR12 at AA rangle: 10-90

GPR12 Polyclonal Antibody

ABP54586-02ml 0.2ml
EUR 414
  • Immunogen information: Synthesized peptide derived from the N-terminal region of human GPR12 at AA rangle: 10-90
  • Applications tips:
Description: A polyclonal antibody for detection of GPR12 from Human. This GPR12 antibody is for WB, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the N-terminal region of human GPR12 at AA rangle: 10-90

GPR12 Conjugated Antibody

C44910 100ul
EUR 397

GPR12 Polyclonal Antibody

ES5585-100ul 100ul
EUR 279
Description: A Rabbit Polyclonal antibody against GPR12 from Human. This antibody is tested and validated for WB, ELISA, IF, WB, ELISA

GPR12 Polyclonal Antibody

ES5585-50ul 50ul
EUR 207
Description: A Rabbit Polyclonal antibody against GPR12 from Human. This antibody is tested and validated for WB, ELISA, IF, WB, ELISA

GPR12 siRNA

20-abx918428
  • EUR 551.00
  • EUR 732.00
  • 15 nmol
  • 30 nmol
  • Shipped within 5-10 working days.

GPR12 siRNA

20-abx918427
  • EUR 551.00
  • EUR 732.00
  • 15 nmol
  • 30 nmol
  • Shipped within 5-10 working days.

GPR12 siRNA

20-abx902268
  • EUR 551.00
  • EUR 732.00
  • 15 nmol
  • 30 nmol
  • Shipped within 5-10 working days.

GPR12 Antibody, HRP conjugated

1-CSB-PA009742LB01HU
  • EUR 317.00
  • EUR 335.00
  • 100ug
  • 50ug
  • Form: Liquid
  • Buffer: Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 >95%, Protein G purified
Description: A polyclonal antibody against GPR12. Recognizes GPR12 from Human. This antibody is HRP conjugated. Tested in the following application: ELISA

GPR12 Antibody, FITC conjugated

1-CSB-PA009742LC01HU
  • EUR 317.00
  • EUR 335.00
  • 100ug
  • 50ug
  • Form: Liquid
  • Buffer: Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 >95%, Protein G purified
Description: A polyclonal antibody against GPR12. Recognizes GPR12 from Human. This antibody is FITC conjugated. Tested in the following application: ELISA

GPR12 Antibody, Biotin conjugated

1-CSB-PA009742LD01HU
  • EUR 317.00
  • EUR 335.00
  • 100ug
  • 50ug
  • Form: Liquid
  • Buffer: Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 >95%, Protein G purified
Description: A polyclonal antibody against GPR12. Recognizes GPR12 from Human. This antibody is Biotin conjugated. Tested in the following application: ELISA

GPR12 Blocking Peptide

DF2721-BP 1mg
EUR 195

GPR12 Blocking Peptide

20-abx062679
  • EUR 272.00
  • EUR 411.00
  • 1 mg
  • 5 mg
  • Shipped within 5-10 working days.

Polyclonal GPR12 Antibody (Extracellular Domain)

APR16480G 0.05mg
EUR 484
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human GPR12 (Extracellular Domain). This antibody is tested and proven to work in the following applications:

Polyclonal GPR12 Antibody (N-Terminus)

APR16481G 0.05mg
EUR 484
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human GPR12 (N-Terminus). This antibody is tested and proven to work in the following applications:

Polyclonal Goat anti-GST α-form

GST-ANTI-1 50 uL
EUR 280

Polyclonal Goat anti-GST μ-form

GST-ANTI-2 50 uL
EUR 280

Polyclonal Goat anti-GST p-form

GST-ANTI-3 50 uL
EUR 280

Rat GPR12 shRNA Plasmid

20-abx986567
  • EUR 801.00
  • EUR 1121.00
  • 150 µg
  • 300 µg
  • Shipped within 15-20 working days.

Human GPR12 shRNA Plasmid

20-abx951900
  • EUR 801.00
  • EUR 1121.00
  • 150 µg
  • 300 µg
  • Shipped within 15-20 working days.

Mouse GPR12 shRNA Plasmid

20-abx970621
  • EUR 801.00
  • EUR 1121.00
  • 150 µg
  • 300 µg
  • Shipped within 15-20 working days.

GPR12 ELISA KIT|Human

EF005024 96 Tests
EUR 689

GPR12 Recombinant Protein (Rat)

RP203285 100 ug Ask for price

GPR12 Recombinant Protein (Rat)

RP203288 100 ug Ask for price

GPR12 Recombinant Protein (Human)

RP061035 100 ug Ask for price

GPR12 Recombinant Protein (Mouse)

RP139421 100 ug Ask for price

GPR12 Recombinant Protein (Mouse)

RP139424 100 ug Ask for price

Polyclonal GPR12 Antibody - C-terminal region

APR16482G 0.05mg
EUR 528
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human GPR12 - C-terminal region. This antibody is tested and proven to work in the following applications:

G Protein Coupled Receptor 12 (GPR12) Antibody

20-abx009208
  • EUR 300.00
  • EUR 439.00
  • EUR 189.00
  • 100 ul
  • 200 ul
  • 30 ul
  • Shipped within 5-10 working days.

G Protein Coupled Receptor 12 (GPR12) Antibody

20-abx324375
  • EUR 314.00
  • EUR 244.00
  • 100 ug
  • 50 ug
  • Shipped within 5-10 working days.

G Protein Coupled Receptor 12 (GPR12) Antibody

20-abx338465
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug
  • Shipped within 5-10 working days.

G Protein Coupled Receptor 12 (GPR12) Antibody

20-abx147559
  • EUR 425.00
  • EUR 342.00
  • 100 ug
  • 50 ug
  • Shipped within 5-10 working days.

GPR12 ORF Vector (Human) (pORF)

ORF020346 1.0 ug DNA
EUR 405

Gpr12 ORF Vector (Mouse) (pORF)

ORF046475 1.0 ug DNA
EUR 506

Gpr12 ORF Vector (Mouse) (pORF)

ORF046476 1.0 ug DNA
EUR 506

Gpr12 ORF Vector (Rat) (pORF)

ORF067763 1.0 ug DNA
EUR 506

Gpr12 ORF Vector (Rat) (pORF)

ORF067764 1.0 ug DNA
EUR 506

G Protein Coupled Receptor 12 (GPR12) Antibody (HRP)

20-abx335850
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug
  • Shipped within 5-10 working days.

G Protein Coupled Receptor 12 (GPR12) Antibody (FITC)

20-abx335851
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug
  • Shipped within 5-10 working days.

G Protein Coupled Receptor 12 (GPR12) Antibody (Biotin)

20-abx335852
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug
  • Shipped within 5-10 working days.

GPR12 sgRNA CRISPR Lentivector set (Human)

K0889201 3 x 1.0 ug
EUR 339

Gpr12 sgRNA CRISPR Lentivector set (Rat)

K7010601 3 x 1.0 ug
EUR 339

Gpr12 sgRNA CRISPR Lentivector set (Mouse)

K3382801 3 x 1.0 ug
EUR 339

GPR12 Protein Vector (Human) (pPB-C-His)

PV081381 500 ng
EUR 552

GPR12 Protein Vector (Human) (pPB-N-His)

PV081382 500 ng
EUR 552

GPR12 Protein Vector (Human) (pPM-C-HA)

PV081383 500 ng
EUR 552

GPR12 Protein Vector (Human) (pPM-C-His)

PV081384 500 ng
EUR 552

GPR12 sgRNA CRISPR Lentivector (Human) (Target 1)

K0889202 1.0 ug DNA
EUR 154

GPR12 sgRNA CRISPR Lentivector (Human) (Target 2)

K0889203 1.0 ug DNA
EUR 154

GPR12 sgRNA CRISPR Lentivector (Human) (Target 3)

K0889204 1.0 ug DNA
EUR 154

Gpr12 sgRNA CRISPR Lentivector (Rat) (Target 1)

K7010602 1.0 ug DNA
EUR 154

Gpr12 sgRNA CRISPR Lentivector (Rat) (Target 2)

K7010603 1.0 ug DNA
EUR 154

Gpr12 sgRNA CRISPR Lentivector (Rat) (Target 3)

K7010604 1.0 ug DNA
EUR 154

Gpr12 sgRNA CRISPR Lentivector (Mouse) (Target 1)

K3382802 1.0 ug DNA
EUR 154

Gpr12 sgRNA CRISPR Lentivector (Mouse) (Target 2)

K3382803 1.0 ug DNA
EUR 154

Gpr12 sgRNA CRISPR Lentivector (Mouse) (Target 3)

K3382804 1.0 ug DNA
EUR 154

GPR12 Protein Vector (Mouse) (pPB-C-His)

PV185898 500 ng
EUR 603

GPR12 Protein Vector (Mouse) (pPB-N-His)

PV185899 500 ng
EUR 603

GPR12 Protein Vector (Mouse) (pPM-C-HA)

PV185900 500 ng
EUR 603

GPR12 Protein Vector (Mouse) (pPM-C-His)

PV185901 500 ng
EUR 603

GPR12 Protein Vector (Mouse) (pPB-C-His)

PV185902 500 ng
EUR 603

GPR12 Protein Vector (Mouse) (pPB-N-His)

PV185903 500 ng
EUR 603

GPR12 Protein Vector (Mouse) (pPM-C-HA)

PV185904 500 ng
EUR 603

GPR12 Protein Vector (Mouse) (pPM-C-His)

PV185905 500 ng
EUR 603

GPR12 Protein Vector (Rat) (pPB-C-His)

PV271050 500 ng
EUR 603

GPR12 Protein Vector (Rat) (pPB-N-His)

PV271051 500 ng
EUR 603

GPR12 Protein Vector (Rat) (pPM-C-HA)

PV271052 500 ng
EUR 603

GPR12 Protein Vector (Rat) (pPM-C-His)

PV271053 500 ng
EUR 603

GPR12 Protein Vector (Rat) (pPB-C-His)

PV271054 500 ng
EUR 603

GPR12 Protein Vector (Rat) (pPB-N-His)

PV271055 500 ng
EUR 603

GPR12 Protein Vector (Rat) (pPM-C-HA)

PV271056 500 ng
EUR 603

GPR12 Protein Vector (Rat) (pPM-C-His)

PV271057 500 ng
EUR 603

Gpr12 3'UTR Luciferase Stable Cell Line

TU205326 1.0 ml Ask for price

GPR12 3'UTR GFP Stable Cell Line

TU059144 1.0 ml
EUR 1394

Gpr12 3'UTR GFP Stable Cell Line

TU255326 1.0 ml Ask for price

GPR12 3'UTR Luciferase Stable Cell Line

TU009144 1.0 ml
EUR 1394

Gpr12 3'UTR Luciferase Stable Cell Line

TU108969 1.0 ml Ask for price

Gpr12 3'UTR GFP Stable Cell Line

TU158969 1.0 ml Ask for price

GPR12 Lentiviral Vector (Rat) (CMV) (pLenti-GIII-CMV)

LV652531 1.0 ug DNA
EUR 682

GPR12 Lentiviral Vector (Rat) (UbC) (pLenti-GIII-UbC)

LV652535 1.0 ug DNA
EUR 682

GPR12 Lentiviral Vector (Rat) (EF1a) (pLenti-GIII-EF1a)

LV652536 1.0 ug DNA
EUR 682

GPR12 Lentiviral Vector (Rat) (CMV) (pLenti-GIII-CMV)

LV626605 1.0 ug DNA
EUR 682