Protokół transfekcji komórek
Na podstawie Maniatis. Rozwiązania2x BBS50 mM BES280 mM NaCl1,5 mM Na2HP04 Chlorek wapnia1 M CaCl2 Nie trzeba wysypywać komórek dzień wcześniej przed faktyczną transfekcją. Utworzyć kompleks DNA: użyć 20 μl […]
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