Liquidambar orientalis Mill. gum extract induces autophagy via PI3K/Akt/mTOR signaling pathway in prostate cancer cells
Endemic species having an area distribution within the southwestern coastal district of Turkey. Styrax liquidus gum (SLG), is a gum-like resinous which exudates in response to harm of the trunk of LOM. The purpose of the research was to analyze the cytotoxic results and the molecular mechanisms of the ethanolic SLG extract in human prostate most cancers cells. GC-MS evaluation was carried out to establish the risky compound composition. Cytotoxicity was decided by XTT evaluation.
Apoptosis and necrosis have been evaluated by way of ELISA assay. Autophagic cell loss of life was detected by way of monodansylcadaverine (MDC) staining and by measuring the degrees of LC3I and LC3II. The protein ranges of p-PI3K, p-Akt and p-mTOR have been evaluated by western blot evaluation.
Within the current research, it’s proven that the SLG extract containing a substantial quantity of ravidomycin derivate induced autophagic cell loss of life in prostate most cancers cells by way of inhibiting the PI3K/Akt/mTOR pathway.
Osteopontin Regulates Endometrial Stromal Cell Migration in Endometriosis by the PI3K Pathway : Osteopontin Regulates Endometrial Cell Migration in Endometriosis
Endometriosis is usually characterised as a tumor-like illness due to its potential for distant metastasis and native tissue invasion, whereas whether or not osteopontin (OPN) performs a job within the pathogenesis of endometriosis has not been completely investigated.
We investigated the expression of OPN, urokinase plasminogen activator (uPA), phosphatidylinositol three kinase (PI3K), and phospho-PI3 kinase (p-PI3K) in endometrial stromal cells (ESCs). The serum focus of OPN was decided by enzyme-linked immunosorbent assays (ELISA). OPN was downregulated to discover the corresponding change of uPA, p-PI3K, F-actin, and α-tubulin.
The expression of OPN, uPA, PI3K, and p-PI3K was evaluated by western blot and quantitative real-time PCR (RT-qPCR) and the expression of F-actin and α-tubulin was confirmed by immunofluorescence assay.
Description: Dissociated Tumor Cells are a single-cell suspension isolated from solid tumors by enzyme and mechanical separation. The cells are stored in cryopreservation medium.
Description: Dissociated Tumor Cells are a single-cell suspension isolated from solid tumors by enzyme and mechanical separation. The cells are stored in cryopreservation medium.
Description: Dissociated Tumor Cells are a single-cell suspension isolated from solid tumors by enzyme and mechanical separation. The cells are stored in cryopreservation medium.
Description: Human MDA-MB-231 is a human adenocarcinoma cells derived from 51 year old (Caucasian) patient metastatic site. MDA-MB-231 are adherent epithelial like cells when culture with DMEM full medium containing 10% FBS. GFP-MDA-MB-231 cells are selected from puromycin resistant MDA-MB-231 after infected with GFP Expressing lentiviral particles. The cells are shipped in proliferating culture with >90 confluence or in a frozen vial with > 1.0 x 106cells/vial.
Description: Human MDA-MB-231 is a human adenocarcinoma cells derived from 51 year old (Caucasian) patient metastatic site. MDA-MB-231 are adherent epithelial like cells when culture with DMEM full medium containing 10% FBS. RFP-MDA-MB-231 cells are selected from Zeocin resistant MDA-MB-231 cells after infected with RFP expressing lentiviral particles. The cells are shipped in proliferating culture with >90 confluence or in a frozen vial with > 1.0 x 106cells/vial.
Description: Included Diagnoses: Non-small Cell Lung Cancer (Adenocarcinoma (including all subtypes) and Squamous Cell Carcinoma (including all subtypes),) Small Cell Lung Cancer and other neuroendocrine tumors of the lung, Carcinoma of the Lung.
Human Skin Cancer Peripheral Blood Mononuclear Cells
Description: Included Diagnoses: Papillary, Follicular, Medullary and other carcinomas (including all subtypes), Adenocarcinoma (including all subtypes), Squamous Cell Carcinoma (including all subtypes), Neuroendocrine tumors of the thyroid (including all subtypes).
Description: Included Diagnoses: Adenocarcinoma (including all subtypes) and Squamous Cell Carcinoma (including all subtypes), Cholangiocarcinoma, Neuroendocrine tumors of the liver, and Hepatocellular carcinoma.
Human Other Cancer Peripheral Blood Mononuclear Cells
Description: Included Diagnoses: Invasive Breast Cancer [Infiltrative (including all subtypes), Adenocarcinoma (including all subtypes) and Squamous Cell Carcinoma (including all subtypes)], Neuroendocrine tumors of the breast, Carcinoma of the breast. For specimens with assoicated tissue,andnbsp, ER,PR and HER2 are offered as a standard service for all Breast Cancer tissue samples.andnbsp, BRCA screening is also available.
Human Kidney Cancer Peripheral Blood Mononuclear Cells
Description: Included Diagnoses: Adenocarcinoma (including all subtypes) and Squamous Cell Carcinoma (including all subtypes),andnbsp, Neuroendocrine tumors of the Kidney, Renal Cell Carcinoma, Clear Cell Carcinoma and other carcinomas of the kidney, Urothelial tumors of the kidney.
Breast Cancer Cells (a-peptide 18), Peptide Aptamer, unlabeled
Description: Included Diagnoses: Papillary, Follicular, Medullary and other carcinomas (including all subtypes), Adenocarcinoma (including all subtypes), Squamous Cell Carcinoma (including all subtypes), Neuroendocrine tumors of the thyroid (including all subtypes).
Human Uterine Cancer Peripheral Blood Mononuclear Cells
Description: Included Diagnoses: Adenocarcinoma (including all subtypes) and Squamous Cell Carcinoma (including all subtypes), Neuroendocrine tumors of the uterus, Carcinoma of the uterus.
Human Bladder Cancer Peripheral Blood Mononuclear Cells
Description: Included Diagnoses: Urothelial Tumors of the bladder, Adenocarcinoma (including all subtypes) and Squamous Cell Carcinoma (including all subtypes), Neuroendocrine tumors of the bladder, Carcinoma of the bladder.
Human Stomach Cancer Peripheral Blood Mononuclear Cells
Description: Included Diagnoses: Adenocarcinoma (including all subtypes) and Squamous Cell Carcinoma (including all subtypes), Neuroendocrine tumors of the stomach, Carcinoma of the stomach.
Human Ovarian Cancer Peripheral Blood Mononuclear Cells
Description: Included Diagnoses: Serous and other carcinomas of the ovary, Endometrial adenocarcinoma (including all subtypes) and Squamous Cell Carcinoma (including all subtypes),Neuroendocrine tumors of the ovary, Brenner Tumors.
Renal Cancer Associated Fibroblasts, 1,000,000 Cells, cryopreserved
Description: Included Diagnoses: Squamous Cell Carcinoma (including all subtypes) and Adenocarcinoma (including all subtypes), Carcinoma of the cervix.
Human Prostate Cancer Peripheral Blood Mononuclear Cells
Description: Included Diagnoses: Adenocarcinoma (including all subtypes) and Squamous Cell Carcinoma (including all subtypes), Neuroendocrine tumors of the prostate, Carcinoma of the prostate.
Description: Included Diagnoses: Included Diagnoses: Adenocarcinoma (including all subtypes) and Squamous Cell Carcinoma (including all subtypes), Neuroendocrine tumors of the colon, rectus and anus, Carcinoma of the colon, rectus and anus.
Human Pancreatic Cancer Peripheral Blood Mononuclear Cells
Description: Included Diagnoses: Adenocarcinoma (including all subtypes) and Squamous Cell Carcinoma (including all subtypes), Neuroendocrine tumors of the Pancreas, Carcinoma of the Pancreas.
Human Esophageal Cancer Peripheral Blood Mononuclear Cells
Description: Included Diagnoses: Adenocarcinoma (including all subtypes) and Squamous Cell Carcinoma (including all subtypes), Neuroendocrine tumors of the esophagus, Carcinoma of the esophagus.
Immortalized Human Cancer Associated Fibroblast Cells - SV40
Description: Included Diagnoses: Squamous Cell Carcinoma (including all subtypes) and Adenocarcinoma (including all subtypes), Neuroendocrine tumors of the Head and Neck, Carcinoma of the Head and Neck.
Human Cancer Tissue Preparation Buffer 5: Cervical Tumor Cells
The proliferation and migration skills of ESCs have been investigated by CCK8, transwell, and wound scratch assays. Endometrial OPN, p-PI3K, and uPA expressions and serum OPN ranges have been elevated in sufferers with endometriosis in contrast with the management. The expressions of p-PI3K, uPA, and α-tubulin have been decreased by siRNA-OPN interference in ectopic ESCs.
Activation and inhibition of the PI3K pathway apparently upregulate and downregulate uPA expression. Knockdown of OPN and inhibition of the PI3K pathway remarkably inhibited cell migration in ectopic ESCs.
In the meantime, activation of the PI3K pathway promoted the migration means of ectopic ESCs. OPN might regulate the expression of uPA by the PI3K sign pathway to have an effect on the migration means of ESCs, indicating that OPN, uPA, and the PI3K pathway could also be potential targets for interrupting improvement of endometriosis.
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