Leprosy transmission in endemic and non-endemic areas based on the profile of antibody response of PGL-1 and PCR detection of Mycobacterium leprae DNA from nasal swab among healthy children of East Java, Indonesia
Background: East Java has become one of the provinces that have higher prevalence of leprosy, especially in the coastal region. Environment has also influenced for leprosy transmission and early detection could reduce the incidence rate of new leprosy cases. Epidemiological studies of leprosy in children can give an illustration of the important aspects of the environment. Presence of Mycobacterium leprae (M. leprae ) DNA in nasal swabs and seropositivity level among them can describe M. leprae exposure in that area.
Objective: Analyzing PCR from nasal swab and seropositivity level among elementary school children between northern coast and southern coast of East Java province.
Methods: Five hundred and thirty children in Pacitan and Lamongan were involved. Both areas are representation of northern and southern coastal region in East Java Province. After clinical examination, nasal swab and blood samples were obtained. ELISA test was performed to measure the titer of IgM anti Phenolic Glicolipid-1 (PGL-1) antibody then continued by PCR to detect M. leprae DNA.
Results: From 301 students in Pacitan, 25 students (8.3%) are sero-positives and 9 students (2.9%) are PCR positives. from 229 students in Lamongan, 110 (48,3%) students are sero-positives and 49 students (21.4%) are PCR positives. Both are analyzed by Chi-Square , and from the PCR and ELISA, it concluded that there are statistically significant differences between the two regions.
Conclusion: From study above shows that in the northern coast of East Java, incidence of subclinical leprosy is still high, it means that in this area still has a high risk of new cases of leprosy in the future and early detection tools need to performed as a preventative measure.
Rar-Related Orphan Receptor B (RORB) Antibody
20-abx115046
Abbexa
Shipped within 5-10 working days.
Rar-Related Orphan Receptor C (RORC) Antibody
20-abx115047
Abbexa
Shipped within 5-10 working days.
RAR Related Orphan Receptor Alpha (RORA) Antibody
20-abx119555
Abbexa
EUR 314.00
EUR 98.00
EUR 398.00
EUR 495.00
100 ug
10 ug
200 ug
300 µg
Shipped within 5-10 working days.
Intermediate Filament Family Orphan 1 (IFFO1) Antibody
20-abx113262
Abbexa
Shipped within 5-10 working days.
Neuron Derived Orphan Receptor 1 (NOR1) Antibody
20-abx130085
Abbexa
EUR 453.00
EUR 133.00
EUR 1302.00
EUR 620.00
EUR 342.00
100 ug
10 ug
1 mg
200 ug
50 ug
Shipped within 5-7 working days.
RAR Related Orphan Receptor Alpha (RORa) Antibody
20-abx103508
Abbexa
EUR 314.00
EUR 133.00
EUR 815.00
EUR 425.00
EUR 272.00
100 ug
10 ug
1 mg
200 ug
50 ug
Shipped within 5-7 working days.
RAR Related Orphan Receptor Gamma (RORg) Antibody
20-abx103509
Abbexa
EUR 425.00
EUR 133.00
EUR 1205.00
EUR 578.00
EUR 328.00
100 ug
10 ug
1 mg
200 ug
50 ug
Shipped within 5-7 working days.
Intermediate Filament Family Orphan 1 (IFFO1) Antibody
abx037159-100ug
Abbexa
100 ug
EUR 391
Shipped within 5-10 working days.
RAR Related Orphan Receptor Gamma (RORG) Antibody
20-abx013514
Abbexa
EUR 314.00
EUR 98.00
EUR 398.00
EUR 495.00
100 ug
10 ug
200 ug
300 µg
Shipped within 5-10 working days.
RAR Related Orphan Receptor Alpha (RORA) Antibody
abx025951-400ul
Abbexa
400 ul
EUR 523
Shipped within 5-10 working days.
RAR Related Orphan Receptor Alpha (RORA) Antibody
abx025951-80l
Abbexa
80 µl
EUR 286
Shipped within 5-10 working days.
RAR Related Orphan Receptor Alpha (RORA) Antibody
20-abx005288
Abbexa
EUR 411.00
EUR 592.00
EUR 182.00
EUR 314.00
100 ul
200 ul
20 ul
50 ul
Shipped within 5-10 working days.
RAR Related Orphan Receptor Beta (RORB) Antibody
abx027769-400ul
Abbexa
400 ul
EUR 523
Shipped within 5-10 working days.
RAR Related Orphan Receptor Beta (RORB) Antibody
abx027769-80l
Abbexa
80 µl
EUR 286
Shipped within 5-10 working days.
Neuron Derived Orphan Receptor 1 (NOR1) Antibody
20-abx173779
Abbexa
RAR Related Orphan Receptor Gamma (RORg) Antibody
20-abx174332
Abbexa
RAR Related Orphan Receptor Gamma (RORg) Antibody
20-abx174333
Abbexa
RAR Related Orphan Receptor Gamma (RORg) Antibody
20-abx178212
Abbexa
RAR Related Orphan Receptor Alpha (RORA) Antibody
20-abx325108
Abbexa
Shipped within 5-10 working days.
RAR Related Orphan Receptor Beta (RORB) Antibody
20-abx214131
Abbexa
Shipped within 5-10 working days.
RAR Related Orphan Receptor Beta (RORB) Antibody
20-abx214132
Abbexa
Shipped within 5-10 working days.
RAR Related Orphan Receptor Alpha (RORA) Antibody
20-abx338779
Abbexa
EUR 411.00
EUR 1845.00
EUR 599.00
EUR 182.00
EUR 300.00
100 ug
1 mg
200 ug
20 ug
50 ug
Shipped within 5-10 working days.
Intermediate Filament Family Orphan 1 (IFFO1) Antibody
abx234132-100ug
Abbexa
100 ug
EUR 481
Shipped within 5-12 working days.
RAR Related Orphan Receptor Alpha (RORA) Antibody
abx237387-100ug
Abbexa
100 ug
EUR 509
Shipped within 5-12 working days.
RAR Related Orphan Receptor Beta (RORB) Antibody
abx237388-100ug
Abbexa
100 ug
EUR 509
Shipped within 5-12 working days.
RAR Related Orphan Receptor Alpha (RORA) Antibody
20-abx211380
Abbexa
Shipped within 5-10 working days.
Receptor Tyrosine Kinase-Like Orphan Receptor 1 Antibody
20-abx115089
Abbexa
Shipped within 5-10 working days.
RAR Related Orphan Receptor Alpha (RORA) Antibody (HRP)
20-abx336856
Abbexa
EUR 411.00
EUR 1845.00
EUR 599.00
EUR 182.00
EUR 300.00
100 ug
1 mg
200 ug
20 ug
50 ug
Shipped within 5-10 working days.
RAR Related Orphan Receptor Alpha (RORA) Antibody (FITC)
20-abx336857
Abbexa
EUR 411.00
EUR 1845.00
EUR 599.00
EUR 182.00
EUR 300.00
100 ug
1 mg
200 ug
20 ug
50 ug
Shipped within 5-10 working days.
RAR Related Orphan Receptor Alpha (RORA) Antibody (Biotin)
20-abx336858
Abbexa
EUR 411.00
EUR 1845.00
EUR 599.00
EUR 182.00
EUR 300.00
100 ug
1 mg
200 ug
20 ug
50 ug
Shipped within 5-10 working days.
Orphan GPCR SP9155 Agonist P518 (human)
H-5984.0500
Bachem
0.5mg
EUR 490
Description: Sum Formula: C127H195N37O37; CAS# [600171-68-4] net
Orphan GPCR SP9155 Agonist P518 (human)
H-5984.1000
Bachem
1.0mg
EUR 853
Description: Sum Formula: C127H195N37O37; CAS# [600171-68-4] net
Orphan GPCR SP9155 Agonist P550 (mouse)
H-5986.0500
Bachem
0.5mg
EUR 490
Description: Sum Formula: C126H195N37O37; CAS# [600171-70-8] net
Orphan GPCR SP9155 Agonist P550 (mouse)
H-5986.1000
Bachem
1.0mg
EUR 853
Description: Sum Formula: C126H195N37O37; CAS# [600171-70-8] net
Receptor Tyrosine Kinase Like Orphan Receptor 2 (ROR2) Antibody
20-abx128158
Abbexa
EUR 425.00
EUR 133.00
EUR 1205.00
EUR 578.00
EUR 328.00
100 ug
10 ug
1 mg
200 ug
50 ug
Shipped within 5-7 working days.
Receptor Tyrosine Kinase Like Orphan Receptor 2 (ROR2) Antibody
20-abx121709
Abbexa
EUR 300.00
EUR 439.00
EUR 189.00
Shipped within 5-10 working days.
Receptor Tyrosine Kinase Like Orphan Receptor 1 (ROR1) Antibody
20-abx002361
Abbexa
EUR 411.00
EUR 592.00
EUR 182.00
EUR 314.00
100 ul
200 ul
20 ul
50 ul
Shipped within 5-10 working days.
Receptor Tyrosine Kinase Like Orphan Receptor 2 (ROR2) Antibody
abx146445-100ug
Abbexa
100 ug
EUR 391
Shipped within 5-10 working days.
Receptor Tyrosine Kinase Like Orphan Receptor 1 (ROR1) Antibody
abx146446-100ug
Abbexa
100 ug
EUR 391
Shipped within 5-10 working days.
Receptor Tyrosine Kinase Like Orphan Receptor 1 (ROR1) Antibody
20-abx104446
Abbexa
EUR 425.00
EUR 133.00
EUR 1205.00
EUR 578.00
EUR 328.00
100 ug
10 ug
1 mg
200 ug
50 ug
Shipped within 5-7 working days.
Receptor Tyrosine Kinase Like Orphan Receptor 1 (ROR1) Antibody
20-abx104447
Abbexa
EUR 439.00
EUR 133.00
EUR 1233.00
EUR 592.00
EUR 328.00
100 ug
10 ug
1 mg
200 ug
50 ug
Shipped within 5-7 working days.
Receptor Tyrosine Kinase Like Orphan Receptor 1 (ROR1) Antibody
abx033600-400ul
Abbexa
400 ul
EUR 523
Shipped within 5-10 working days.
Receptor Tyrosine Kinase Like Orphan Receptor 1 (ROR1) Antibody
abx033600-80l
Abbexa
80 µl
EUR 286
Shipped within 5-10 working days.
Receptor Tyrosine Kinase Like Orphan Receptor 1 (ROR1) Antibody
abx033601-400ul
Abbexa
400 ul
EUR 523
Shipped within 5-10 working days.
Receptor Tyrosine Kinase Like Orphan Receptor 1 (ROR1) Antibody
abx033601-80l
Abbexa
80 µl
EUR 286
Shipped within 5-10 working days.
Receptor Tyrosine Kinase Like Orphan Receptor 2 (ROR2) Antibody
abx033602-400ul
Abbexa
400 ul
EUR 523
Shipped within 5-10 working days.
Receptor Tyrosine Kinase Like Orphan Receptor 2 (ROR2) Antibody
abx033602-80l
Abbexa
80 µl
EUR 286
Shipped within 5-10 working days.
Receptor Tyrosine Kinase Like Orphan Receptor 2 (ROR2) Antibody
abx033603-400ul
Abbexa
400 ul
EUR 523
Shipped within 5-10 working days.
Receptor Tyrosine Kinase Like Orphan Receptor 2 (ROR2) Antibody
abx033603-80l
Abbexa
80 µl
EUR 286
Shipped within 5-10 working days.
Receptor Tyrosine Kinase Like Orphan Receptor 2 (ROR2) Antibody
abx033604-400ul
Abbexa
400 ul
EUR 523
Shipped within 5-10 working days.
Receptor Tyrosine Kinase Like Orphan Receptor 2 (ROR2) Antibody
abx033604-80l
Abbexa
80 µl
EUR 286
Shipped within 5-10 working days.
Receptor Tyrosine Kinase Like Orphan Receptor 1 (ROR1) Antibody
abx011486-100ul
Abbexa
100 ul
EUR 411
Shipped within 5-10 working days.
Receptor Tyrosine Kinase Like Orphan Receptor 2 (ROR2) Antibody
20-abx004297
Abbexa
EUR 411.00
EUR 592.00
EUR 182.00
EUR 314.00
100 ul
200 ul
20 ul
50 ul
Shipped within 5-10 working days.
Receptor Tyrosine Kinase Like Orphan Receptor 1 (ROR1) Antibody
20-abx174350
Abbexa
Receptor Tyrosine Kinase Like Orphan Receptor 2 (ROR2) Antibody
20-abx328028
Abbexa
Shipped within 5-10 working days.
Receptor Tyrosine Kinase Like Orphan Receptor 2 (ROR2) Antibody
abx218339-100ug
Abbexa
100 ug
EUR 439
Shipped within 5-10 working days.
Receptor Tyrosine Kinase Like Orphan Receptor 2 (ROR2) Antibody
20-abx334125
Abbexa
EUR 411.00
EUR 1845.00
EUR 599.00
EUR 182.00
EUR 300.00
100 ug
1 mg
200 ug
20 ug
50 ug
Shipped within 5-10 working days.
Receptor Tyrosine Kinase Like Orphan Receptor 2 (ROR2) Antibody
abx448581-100ug
Abbexa
100 ug
EUR 523
Shipped within 5-12 working days.
Receptor Tyrosine Kinase Like Orphan Receptor 1 (ROR1) Antibody
abx449254-100ug
Abbexa
100 ug
EUR 551
Shipped within 5-12 working days.
Receptor Tyrosine Kinase Like Orphan Receptor 1 (ROR1) Antibody
abx449272-100ug
Abbexa
100 ug
EUR 551
Shipped within 5-12 working days.
Receptor Tyrosine Kinase Like Orphan Receptor 1 (ROR1) Antibody
abx431844-200ul
Abbexa
200 ul
EUR 384
Shipped within 1-3 working days.
Receptor Tyrosine Kinase Like Orphan Receptor 1 (ROR1) Antibody
abx237386-100ug
Abbexa
100 ug
EUR 509
Shipped within 5-12 working days.
Receptor Tyrosine Kinase Like Orphan Receptor 1 (ROR1) Antibody
20-abx212778
Abbexa
Shipped within 5-10 working days.
Receptor Tyrosine Kinase Like Orphan Receptor 1 (ROR1) Antibody
20-abx212779
Abbexa
Shipped within 5-10 working days.
Neuron Derived Orphan Receptor 1 (NOR1) Polyclonal Antibody (Rat)
4-PAC675Ra01
Cloud-Clone
EUR 259.00
EUR 2708.00
EUR 670.00
EUR 328.00
EUR 219.00
100ul
10ml
1ml
200ul
20ul
Sequence of the immunogen: NOR1 (Glu29~Pro145) Buffer composition: PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Description: A Rabbit polyclonal antibody against Rat Neuron Derived Orphan Receptor 1 (NOR1)
RAR Related Orphan Receptor Gamma (RORG) Protein
20-abx261968
Abbexa
EUR 4490.00
EUR 328.00
EUR 230.00
Shipped within 5-10 working days.
Recombinant Human RAR-Related Orphan Receptor C
7-06163
CHI Scientific
5µg
Ask for price
Recombinant Human RAR-Related Orphan Receptor C
7-06164
CHI Scientific
20µg
Ask for price
Recombinant Human RAR-Related Orphan Receptor C
7-06165
CHI Scientific
1mg
Ask for price
Recombinant RAR Related Orphan Receptor Alpha (RORa)
4-RPD947Hu01
Cloud-Clone
EUR 483.49
EUR 232.00
EUR 1538.08
EUR 579.36
EUR 1058.72
EUR 386.00
EUR 3695.20
100 ug
10ug
1 mg
200 ug
500 ug
50ug
5 mg
Uniprot ID: P35398 Buffer composition: PBS, pH 7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. Form: Freeze-dried powder Predicted Molecular Mass (KD): 31.0kDa Isoelectric Point: Inquire
Description: Recombinant Human RAR Related Orphan Receptor Alpha expressed in: E.coli
Recombinant RAR Related Orphan Receptor Gamma (RORg)
4-RPD949Hu01
Cloud-Clone
EUR 488.86
EUR 234.00
EUR 1558.24
EUR 586.08
EUR 1072.16
EUR 390.00
EUR 3745.60
100 ug
10ug
1 mg
200 ug
500 ug
50ug
5 mg
Uniprot ID: P51449 Buffer composition: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300. Form: Freeze-dried powder Predicted Molecular Mass (KD): 32.5kDa Isoelectric Point: 6.6
Description: Recombinant Human RAR Related Orphan Receptor Gamma expressed in: E.coli
Recombinant Neuron Derived Orphan Receptor 1 (NOR1)
4-RPC675Ra01
Cloud-Clone
EUR 512.16
EUR 240.00
EUR 1645.60
EUR 615.20
EUR 1130.40
EUR 406.00
EUR 3964.00
100 ug
10ug
1 mg
200 ug
500 ug
50ug
5 mg
Uniprot ID: P51179 Buffer composition: PBS, pH 7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. Form: Freeze-dried powder Predicted Molecular Mass (KD): 16.9kDa Isoelectric Point: Inquire
Description: Recombinant Rat Neuron Derived Orphan Receptor 1 expressed in: E.coli
PHOSPHO2 Phosphatase Orphan-2 Human Recombinant Protein
PROTQ8TCD6
BosterBio
Regular: 10ug
EUR 317
Description: PHOSPHO2 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 265 amino acids (1-241) and having a molecular mass of 30.3kDa.;PHOSPHO2 is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
PHOSPHO1 Phosphatase Orphan-1 Human Recombinant Protein
PROTQ8TCT1
BosterBio
Regular: 10ug
EUR 317
Description: Human Phospho1 Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 295 amino acids and having a molecular mass of 31.3 kDa. The Human Phospho1 is fused to a 14 aa His tag at N-Terminus. Human Phosphocholine Phosphatase is purified by proprietary chromatographic techniques.
Receptor Tyrosine Kinase Like Orphan Receptor 1 (ROR1) Antibody (HRP)
20-abx108873
Abbexa
EUR 411.00
EUR 1845.00
EUR 599.00
EUR 182.00
EUR 300.00
100 ug
1 mg
200 ug
20 ug
50 ug
Shipped within 5-10 working days.
Receptor Tyrosine Kinase Like Orphan Receptor 1 (ROR1) Antibody (Biotin)
20-abx106039
Abbexa
EUR 411.00
EUR 1845.00
EUR 599.00
EUR 182.00
EUR 300.00
100 ug
1 mg
200 ug
20 ug
50 ug
Shipped within 5-10 working days.
Receptor Tyrosine Kinase Like Orphan Receptor 1 (ROR1) Antibody (FITC)
20-abx107453
Abbexa
EUR 411.00
EUR 1845.00
EUR 599.00
EUR 182.00
EUR 300.00
100 ug
1 mg
200 ug
20 ug
50 ug
Shipped within 5-10 working days.
Receptor Tyrosine Kinase Like Orphan Receptor 2 (ROR2) Antibody (HRP)
20-abx338152
Abbexa
EUR 411.00
EUR 1845.00
EUR 599.00
EUR 182.00
EUR 300.00
100 ug
1 mg
200 ug
20 ug
50 ug
Shipped within 5-10 working days.
Receptor Tyrosine Kinase Like Orphan Receptor 2 (ROR2) Antibody (FITC)
20-abx338153
Abbexa
EUR 411.00
EUR 1845.00
EUR 599.00
EUR 182.00
EUR 300.00
100 ug
1 mg
200 ug
20 ug
50 ug
Shipped within 5-10 working days.
Receptor Tyrosine Kinase Like Orphan Receptor 2 (ROR2) Antibody (Biotin)
20-abx338154
Abbexa
EUR 411.00
EUR 1845.00
EUR 599.00
EUR 182.00
EUR 300.00
100 ug
1 mg
200 ug
20 ug
50 ug
Shipped within 5-10 working days.
Receptor Tyrosine Kinase Like Orphan Receptor 1 (ROR1) Antibody (APC)
abx449264-100ug
Abbexa
100 ug
EUR 606
Shipped within 5-12 working days.
Receptor Tyrosine Kinase Like Orphan Receptor 1 (ROR1) Antibody (Biotin)
abx449265-100ug
Abbexa
100 ug
EUR 606
Shipped within 5-12 working days.
Receptor Tyrosine Kinase Like Orphan Receptor 1 (ROR1) Antibody (FITC)
abx449266-100ug
Abbexa
100 ug
EUR 606
Shipped within 5-12 working days.
Receptor Tyrosine Kinase Like Orphan Receptor 1 (ROR1) Antibody (HRP)
abx449267-100ug
Abbexa
100 ug
EUR 592
Shipped within 5-12 working days.
Receptor Tyrosine Kinase Like Orphan Receptor 1 (ROR1) Antibody (PerCP)
abx449269-100ug
Abbexa
100 ug
EUR 606
Shipped within 5-12 working days.
Receptor Tyrosine Kinase Like Orphan Receptor 1 (ROR1) Antibody (RPE)
abx449270-100ug
Abbexa
100 ug
EUR 606
Shipped within 5-12 working days.
Receptor Tyrosine Kinase Like Orphan Receptor 1 (ROR1) Antibody (Streptavidin)
abx449271-100ug
Abbexa
100 ug
EUR 606
Shipped within 5-12 working days.
Receptor Tyrosine Kinase Like Orphan Receptor 1 (ROR1) Antibody (APC)
abx449282-100ug
Abbexa
100 ug
EUR 606
Shipped within 5-12 working days.
Receptor Tyrosine Kinase Like Orphan Receptor 1 (ROR1) Antibody (Biotin)
abx449283-100ug
Abbexa
100 ug
EUR 606
Shipped within 5-12 working days.
Receptor Tyrosine Kinase Like Orphan Receptor 1 (ROR1) Antibody (FITC)
abx449284-100ug
Abbexa
100 ug
EUR 606
Shipped within 5-12 working days.
Receptor Tyrosine Kinase Like Orphan Receptor 1 (ROR1) Antibody (HRP)
abx449285-100ug
Abbexa
100 ug
EUR 592
Shipped within 5-12 working days.
Receptor Tyrosine Kinase Like Orphan Receptor 1 (ROR1) Antibody (PerCP)
abx449287-100ug
Abbexa
100 ug
EUR 606
Shipped within 5-12 working days.
Receptor Tyrosine Kinase Like Orphan Receptor 1 (ROR1) Antibody (RPE)
abx449288-100ug
Abbexa
100 ug
EUR 606
Shipped within 5-12 working days.
Receptor Tyrosine Kinase Like Orphan Receptor 1 (ROR1) Antibody (Streptavidin)
abx449289-100ug
Abbexa
100 ug
EUR 606
Shipped within 5-12 working days.
Receptor Tyrosine Kinase Like Orphan Receptor 2 (ROR2) Antibody (ALP)
abx445965-100ug
Abbexa
100 ug
EUR 578
Shipped within 5-12 working days.
Receptor Tyrosine Kinase Like Orphan Receptor 2 (ROR2) Antibody (APC)
abx445966-100ug
Abbexa
100 ug
EUR 578
Shipped within 5-12 working days.
Receptor Tyrosine Kinase Like Orphan Receptor 2 (ROR2) Antibody (Biotin)
abx445967-100ug
Abbexa
100 ug
EUR 578
Shipped within 5-12 working days.
Receptor Tyrosine Kinase Like Orphan Receptor 2 (ROR2) Antibody (FITC)
abx445968-100ug
Abbexa
100 ug
EUR 565
Shipped within 5-12 working days.
Receptor Tyrosine Kinase Like Orphan Receptor 2 (ROR2) Antibody (HRP)
abx445969-100ug
Abbexa
100 ug
EUR 565
Shipped within 5-12 working days.
Receptor Tyrosine Kinase Like Orphan Receptor 2 (ROR2) Antibody (PerCP)
abx445971-100ug
Abbexa
100 ug
EUR 578
Shipped within 5-12 working days.
Optimized broad-range real-time PCR -based method for bacterial screening of platelet concentrates
Bacterial contamination of blood components remains a major challenge in transfusion medicine, particularly, platelet concentrates (PCs) due to the storage conditions that support bacterial proliferation. In this study, we develop a rapid, sensitive and specific real-time PCR protocol for bacterial screening of PCs. An internally controlled real-time PCR-based method was optimized and validated with our proprietary 16S Universal PCR Master Mix (IBMP/Fiocruz ), which targets a conserved region of the bacterial 16S rRNA gene. Nonspecific background DNA was completely eliminated by treating the PCR Master Mix with ethidium monoazide (EMA).
A lower limit of detection was observed for 10 genome equivalents with an observed Ct value of 34±1.07 in calibration curve generated with 10-fold serial dilutions of E. coli DNA. The turnaround time for processing, including microbial DNA purification, was approximately 4 hours. The developed method showed a high sensitivity with no non-specific amplification and a lower time-to-detection than traditional microbiological methods, demonstrating it to be an efficient means of screening pre-transfusion PCs.
Evaluation of sample pooling for diagnosis of COVID-19 by Real time PCR – A resource saving combat strategy
Background& objectives: Although about 80% of COVID-19 cases are reported to be mild, the remaining 20% of cases often result in a severe disease with the potential of crushing already overstrained health care services. There has been sustainable growth of COVID-19 cases worldwide since mid-May 2020. In order to keep tabs on community transmission of COVID-19 infection screening of the samples from large population is needed which includes asymptomatic/symptomatic individuals along with migrant population. This requires extra resources, man power and time for detection of SARS-CoV-2by RT-PCR . In current scenario pooled sample testingstrategy advocated by ICMR, New Delhiis a new approach which is very promising in resource limited settings. In this study we have evaluated pooled strategy in terms of accurate testing results, utilization of consumables and identification of border line positive cases.
Materials & methods: Between April-June 2020 we performed COVID-19 testing by RTPCR from areas with varying prevalence of population referred to COVID laboratory, Dr RMLIMS , Lucknow. In a first step the samples are collated into pools of 5 or 10.These pools are tested by RT-PCR . Negative Pools were reported as negative whereas positive pools of 5 and 10 are then deconvoluted and each sample is tested individually.
Results: In the present study, we tested 4620 samples in 462 pools of 10 and 14940 samples in 2990 pools of 5. Among 10 samples pool 61 (13%) pools flagged positive in first step. In second step among 61 pools (610 samples) deconvoluted strategy was followed in which 72 individual samples came positive. Pooled-sample testing strategy helps saves substantial resources and time during surge testing and enhanced pandemic surveillance. This approach requires around 76% to 93% less tests done in low to moderate prevalence settings and group sizes up to 5-10 in a population, compared to individual testing.
Conclusions: Pooled-sample PCR analysis strategies can save substantial resources and time for COVID-19 mass testing in comparison to individual testing without compromising the result outcome of test. In particular, pooled-sample approach can facilitate mass screening in early coming stages of COVID-19 outbreaks, especially in low- and middle-income settings and control the spread by meticulous testing of all risk groups. This article is protected by copyright. All rights reserved.
MULTIPLEX KIT PCR Babesia & Theileria PCR kit
PCR-MPX401-96D
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Description: An conventional PCR kit for detection of MULTIPLEX KIT PCR Babesia & Theileria
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Chlamydia pneumoniae PCR kit
PCR-H439-48D
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PCR-H439-96D
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Pneumocystis jiroveci PCR kit
PCR-H440-48D
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PCR-H440-96D
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Actinobacillus actinomycetemcomitans PCR kit
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PCR-H446-96D
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PCR-H447-48D
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