Leprosy transmission in endemic and non-endemic areas based on the profile of antibody response of PGL-1 and PCR detection of Mycobacterium leprae DNA from nasal swab among healthy children of East Java, Indonesia

Background: East Java has become one of the provinces that have higher prevalence of leprosy, especially in the coastal region. Environment has also influenced for leprosy transmission and early detection could reduce the incidence rate of new leprosy cases. Epidemiological studies of leprosy in children can give an illustration of the important aspects of the environment. Presence of Mycobacterium leprae (M. leprae) DNA in nasal swabs and seropositivity level among them can describe M. leprae exposure in that area.

Objective: Analyzing PCR from nasal swab and seropositivity level among elementary school children between northern coast and southern coast of East Java province.

Methods: Five hundred and thirty children in Pacitan and Lamongan were involved. Both areas are representation of northern and southern coastal region in East Java Province. After clinical examination, nasal swab and blood samples were obtained. ELISA test was performed to measure the titer of IgM anti Phenolic Glicolipid-1 (PGL-1) antibody then continued by PCR to detect M. leprae DNA.

Results: From 301 students in Pacitan, 25 students (8.3%) are sero-positives and 9 students (2.9%) are PCR positives. from 229 students in Lamongan, 110 (48,3%) students are sero-positives and 49 students (21.4%) are PCR positives. Both are analyzed by Chi-Square, and from the PCR and ELISA, it concluded that there are statistically significant differences between the two regions.

Conclusion: From study above shows that in the northern coast of East Java, incidence of subclinical leprosy is still high, it means that in this area still has a high risk of new cases of leprosy in the future and early detection tools need to performed as a preventative measure.

RAR Related Orphan Receptor Beta (RORB) Antibody

abx027769-400ul 400 ul
EUR 627.6

RAR Related Orphan Receptor Beta (RORB) Antibody

abx027769-80l 80 µl
EUR 343.2

RAR Related Orphan Receptor Alpha (RORA) Antibody

abx237387-100ug 100 ug
EUR 610.8

RAR Related Orphan Receptor Alpha (RORa) Antibody

20-abx103508
  • EUR 376.80
  • EUR 159.60
  • EUR 978.00
  • EUR 510.00
  • EUR 326.40
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

RAR Related Orphan Receptor Gamma (RORg) Antibody

20-abx103509
  • EUR 510.00
  • EUR 159.60
  • EUR 1446.00
  • EUR 693.60
  • EUR 393.60
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Neuron Derived Orphan Receptor 1 (NOR1) Antibody

20-abx173779
  • EUR 1095.60
  • EUR 560.40
  • 1 mg
  • 200 ug

RAR Related Orphan Receptor Gamma (RORg) Antibody

20-abx174332
  • EUR 1028.40
  • EUR 526.80
  • 1 mg
  • 200 ug

RAR Related Orphan Receptor Gamma (RORg) Antibody

20-abx174333
  • EUR 1095.60
  • EUR 560.40
  • 1 mg
  • 200 ug

RAR Related Orphan Receptor Gamma (RORg) Antibody

20-abx178212
  • EUR 1562.40
  • EUR 744.00
  • 1 mg
  • 200 ug

RAR Related Orphan Receptor Alpha (RORA) Antibody

20-abx211380
  • EUR 493.20
  • EUR 360.00
  • 100 ul
  • 50 ul

RAR Related Orphan Receptor Alpha (RORA) Antibody

20-abx119555
  • EUR 376.80
  • EUR 117.60
  • EUR 477.60
  • EUR 594.00
  • 100 ug
  • 10 ug
  • 200 ug
  • 300 µg

RAR Related Orphan Receptor Alpha (RORA) Antibody

20-abx325108
  • EUR 376.80
  • EUR 292.80
  • 100 ug
  • 50 ug

RAR Related Orphan Receptor Alpha (RORA) Antibody

abx025951-400ul 400 ul
EUR 627.6

RAR Related Orphan Receptor Alpha (RORA) Antibody

abx025951-80l 80 µl
EUR 343.2

Neuron Derived Orphan Receptor 1 (NOR1) Antibody

20-abx130085
  • EUR 543.60
  • EUR 159.60
  • EUR 1562.40
  • EUR 744.00
  • EUR 410.40
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

RAR Related Orphan Receptor Alpha (RORA) Antibody

20-abx338779
  • EUR 493.20
  • EUR 2214.00
  • EUR 718.80
  • EUR 218.40
  • EUR 360.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

RAR Related Orphan Receptor Alpha (RORA) Antibody

20-abx005288
  • EUR 493.20
  • EUR 710.40
  • EUR 218.40
  • EUR 376.80
  • 100 ul
  • 200 ul
  • 20 ul
  • 50 ul

RAR Related Orphan Receptor Gamma (RORG) Antibody

20-abx013514
  • EUR 376.80
  • EUR 117.60
  • EUR 477.60
  • EUR 594.00
  • 100 ug
  • 10 ug
  • 200 ug
  • 300 µg

Intermediate Filament Family Orphan 1 (IFFO1) Antibody

abx234132-100ug 100 ug
EUR 577.2

Intermediate Filament Family Orphan 1 (IFFO1) Antibody

20-abx113262
  • EUR 878.40
  • EUR 477.60
  • 150 ul
  • 50 ul

Intermediate Filament Family Orphan 1 (IFFO1) Antibody

abx037159-100ug 100 ug
EUR 469.2

RAR Related Orphan Receptor Alpha (RORA) Antibody (HRP)

20-abx336856
  • EUR 493.20
  • EUR 2214.00
  • EUR 718.80
  • EUR 218.40
  • EUR 360.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

RAR Related Orphan Receptor Alpha (RORA) Antibody (FITC)

20-abx336857
  • EUR 493.20
  • EUR 2214.00
  • EUR 718.80
  • EUR 218.40
  • EUR 360.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

RAR Related Orphan Receptor Alpha (RORA) Antibody (Biotin)

20-abx336858
  • EUR 493.20
  • EUR 2214.00
  • EUR 718.80
  • EUR 218.40
  • EUR 360.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Neuron Derived Orphan Receptor 1 (NOR1) Polyclonal Antibody

CAU23984-100ul 100ul
EUR 255.4

Neuron Derived Orphan Receptor 1 (NOR1) Polyclonal Antibody

CAU23984-200ul 200ul
EUR 319.2

RAR Related Orphan Receptor Gamma (RORg) Polyclonal Antibody

CAU22965-100ul 100ul
EUR 235.2

RAR Related Orphan Receptor Gamma (RORg) Polyclonal Antibody

CAU22965-200ul 200ul
EUR 294

RAR Related Orphan Receptor Alpha (RORa) Polyclonal Antibody

CAU22966-100ul 100ul
EUR 235.2

RAR Related Orphan Receptor Alpha (RORa) Polyclonal Antibody

CAU22966-200ul 200ul
EUR 294

Receptor Tyrosine Kinase-Like Orphan Receptor 1 Antibody

20-abx115089
  • EUR 878.40
  • EUR 477.60
  • 150 ul
  • 50 ul

Polyclonal Antibody to Neuron Derived Orphan Receptor 1 (NOR1)

PAC675Ra01 100ul
EUR 266

Polyclonal Antibody to RAR Related Orphan Receptor Alpha (RORa)

PAD947Hu01 100ul
EUR 245

Polyclonal Antibody to RAR Related Orphan Receptor Gamma (RORg)

PAD949Hu01 100ul
EUR 245

Polyclonal Antibody to RAR Related Orphan Receptor Gamma (RORg)

PAD949Mu01 100ul
EUR 252

Polyclonal Antibody to RAR Related Orphan Receptor Gamma (RORg)

PAD949Ra01 100ul
EUR 266

Receptor Tyrosine Kinase Like Orphan Receptor 2 (ROR2) Antibody

abx218339-100ug 100 ug
EUR 526.8

Receptor Tyrosine Kinase Like Orphan Receptor 1 (ROR1) Antibody

abx237386-100ug 100 ug
EUR 610.8

Receptor Tyrosine Kinase Like Orphan Receptor 1 (ROR1) Antibody

20-abx104446
  • EUR 510.00
  • EUR 159.60
  • EUR 1446.00
  • EUR 693.60
  • EUR 393.60
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Receptor Tyrosine Kinase Like Orphan Receptor 1 (ROR1) Antibody

20-abx104447
  • EUR 526.80
  • EUR 159.60
  • EUR 1479.60
  • EUR 710.40
  • EUR 393.60
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Receptor Tyrosine Kinase Like Orphan Receptor 1 (ROR1) Antibody

20-abx174350
  • EUR 1028.40
  • EUR 526.80
  • 1 mg
  • 200 ug

Receptor Tyrosine Kinase Like Orphan Receptor 1 (ROR1) Antibody

20-abx212778
  • EUR 493.20
  • EUR 360.00
  • 100 ul
  • 50 ul

Receptor Tyrosine Kinase Like Orphan Receptor 1 (ROR1) Antibody

20-abx212779
  • EUR 493.20
  • EUR 360.00
  • 100 ul
  • 50 ul

Receptor Tyrosine Kinase Like Orphan Receptor 2 (ROR2) Antibody

20-abx121709
  • EUR 360.00
  • EUR 526.80
  • EUR 226.80
  • 100 ul
  • 200 ul
  • 30 ul

Receptor Tyrosine Kinase Like Orphan Receptor 2 (ROR2) Antibody

abx146445-100ug 100 ug
EUR 469.2

Receptor Tyrosine Kinase Like Orphan Receptor 1 (ROR1) Antibody

abx146446-100ug 100 ug
EUR 469.2

Receptor Tyrosine Kinase Like Orphan Receptor 2 (ROR2) Antibody

abx448581-100ug 100 ug
EUR 627.6

Receptor Tyrosine Kinase Like Orphan Receptor 1 (ROR1) Antibody

abx449254-100ug 100 ug
EUR 661.2

Receptor Tyrosine Kinase Like Orphan Receptor 1 (ROR1) Antibody

abx449272-100ug 100 ug
EUR 661.2

Receptor Tyrosine Kinase Like Orphan Receptor 1 (ROR1) Antibody

abx033600-400ul 400 ul
EUR 627.6

Receptor Tyrosine Kinase Like Orphan Receptor 1 (ROR1) Antibody

abx033600-80l 80 µl
EUR 343.2

Receptor Tyrosine Kinase Like Orphan Receptor 1 (ROR1) Antibody

abx033601-400ul 400 ul
EUR 627.6

Receptor Tyrosine Kinase Like Orphan Receptor 1 (ROR1) Antibody

abx033601-80l 80 µl
EUR 343.2

Receptor Tyrosine Kinase Like Orphan Receptor 2 (ROR2) Antibody

abx033602-400ul 400 ul
EUR 627.6

Receptor Tyrosine Kinase Like Orphan Receptor 2 (ROR2) Antibody

abx033602-80l 80 µl
EUR 343.2

Receptor Tyrosine Kinase Like Orphan Receptor 2 (ROR2) Antibody

abx033603-400ul 400 ul
EUR 627.6

Receptor Tyrosine Kinase Like Orphan Receptor 2 (ROR2) Antibody

abx033603-80l 80 µl
EUR 343.2

Receptor Tyrosine Kinase Like Orphan Receptor 2 (ROR2) Antibody

abx033604-400ul 400 ul
EUR 627.6

Receptor Tyrosine Kinase Like Orphan Receptor 2 (ROR2) Antibody

abx033604-80l 80 µl
EUR 343.2

Receptor Tyrosine Kinase Like Orphan Receptor 2 (ROR2) Antibody

20-abx128158
  • EUR 510.00
  • EUR 159.60
  • EUR 1446.00
  • EUR 693.60
  • EUR 393.60
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Receptor Tyrosine Kinase Like Orphan Receptor 2 (ROR2) Antibody

20-abx328028
  • EUR 376.80
  • EUR 292.80
  • 100 ug
  • 50 ug

Receptor Tyrosine Kinase Like Orphan Receptor 2 (ROR2) Antibody

20-abx334125
  • EUR 493.20
  • EUR 2214.00
  • EUR 718.80
  • EUR 218.40
  • EUR 360.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Receptor Tyrosine Kinase Like Orphan Receptor 1 (ROR1) Antibody

abx431844-200ul 200 ul
EUR 460.8

Receptor Tyrosine Kinase Like Orphan Receptor 1 (ROR1) Antibody

20-abx002361
  • EUR 493.20
  • EUR 710.40
  • EUR 218.40
  • EUR 376.80
  • 100 ul
  • 200 ul
  • 20 ul
  • 50 ul

Receptor Tyrosine Kinase Like Orphan Receptor 2 (ROR2) Antibody

20-abx004297
  • EUR 493.20
  • EUR 710.40
  • EUR 218.40
  • EUR 376.80
  • 100 ul
  • 200 ul
  • 20 ul
  • 50 ul

Receptor Tyrosine Kinase Like Orphan Receptor 1 (ROR1) Antibody

abx011486-100ul 100 ul
EUR 493.2

Neuron Derived Orphan Receptor 1 (NOR1) Polyclonal Antibody (Rat)

4-PAC675Ra01
  • EUR 310.80
  • EUR 3249.60
  • EUR 804.00
  • EUR 393.60
  • EUR 262.80
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Rat Neuron Derived Orphan Receptor 1 (NOR1)

Neuron Derived Orphan Receptor 1 (NOR1) Polyclonal Antibody (Rat), PE

4-PAC675Ra01-PE
  • EUR 373.20
  • EUR 3427.20
  • EUR 964.80
  • EUR 471.60
  • EUR 242.40
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Rat Neuron Derived Orphan Receptor 1 (NOR1). This antibody is labeled with PE.

Receptor Tyrosine Kinase Like Orphan Receptor 2 (ROR2) Antibody (ALP)

abx445965-100ug 100 ug
EUR 693.6

Receptor Tyrosine Kinase Like Orphan Receptor 2 (ROR2) Antibody (APC)

abx445966-100ug 100 ug
EUR 693.6

Receptor Tyrosine Kinase Like Orphan Receptor 2 (ROR2) Antibody (HRP)

abx445969-100ug 100 ug
EUR 678

Receptor Tyrosine Kinase Like Orphan Receptor 2 (ROR2) Antibody (RPE)

abx445972-100ug 100 ug
EUR 693.6

Receptor Tyrosine Kinase Like Orphan Receptor 1 (ROR1) Antibody (APC)

abx449264-100ug 100 ug
EUR 727.2

Receptor Tyrosine Kinase Like Orphan Receptor 1 (ROR1) Antibody (HRP)

abx449267-100ug 100 ug
EUR 710.4

Receptor Tyrosine Kinase Like Orphan Receptor 1 (ROR1) Antibody (RPE)

abx449270-100ug 100 ug
EUR 727.2

Receptor Tyrosine Kinase Like Orphan Receptor 1 (ROR1) Antibody (APC)

abx449282-100ug 100 ug
EUR 727.2

Receptor Tyrosine Kinase Like Orphan Receptor 1 (ROR1) Antibody (HRP)

abx449285-100ug 100 ug
EUR 710.4

Receptor Tyrosine Kinase Like Orphan Receptor 1 (ROR1) Antibody (RPE)

abx449288-100ug 100 ug
EUR 727.2

Receptor Tyrosine Kinase Like Orphan Receptor 1 (ROR1) Antibody (HRP)

20-abx108873
  • EUR 493.20
  • EUR 2214.00
  • EUR 718.80
  • EUR 218.40
  • EUR 360.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Receptor Tyrosine Kinase Like Orphan Receptor 2 (ROR2) Antibody (HRP)

20-abx338152
  • EUR 493.20
  • EUR 2214.00
  • EUR 718.80
  • EUR 218.40
  • EUR 360.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Neuron Derived Orphan Receptor 1 (NOR1) Polyclonal Antibody (Rat), APC

4-PAC675Ra01-APC
  • EUR 436.80
  • EUR 4254.00
  • EUR 1176.00
  • EUR 560.40
  • EUR 272.40
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Rat Neuron Derived Orphan Receptor 1 (NOR1). This antibody is labeled with APC.

Neuron Derived Orphan Receptor 1 (NOR1) Polyclonal Antibody (Rat), Cy3

4-PAC675Ra01-Cy3
  • EUR 532.80
  • EUR 5622.00
  • EUR 1518.00
  • EUR 697.20
  • EUR 313.20
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Rat Neuron Derived Orphan Receptor 1 (NOR1). This antibody is labeled with Cy3.

Neuron Derived Orphan Receptor 1 (NOR1) Polyclonal Antibody (Rat), HRP

4-PAC675Ra01-HRP
  • EUR 398.40
  • EUR 3706.80
  • EUR 1039.20
  • EUR 505.20
  • EUR 255.60
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Rat Neuron Derived Orphan Receptor 1 (NOR1). This antibody is labeled with HRP.

Receptor Tyrosine Kinase Like Orphan Receptor 1 (ROR1) Antibody (FITC)

20-abx107453
  • EUR 493.20
  • EUR 2214.00
  • EUR 718.80
  • EUR 218.40
  • EUR 360.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Receptor Tyrosine Kinase Like Orphan Receptor 2 (ROR2) Antibody (FITC)

abx445968-100ug 100 ug
EUR 678

Receptor Tyrosine Kinase Like Orphan Receptor 1 (ROR1) Antibody (FITC)

abx449266-100ug 100 ug
EUR 727.2

Receptor Tyrosine Kinase Like Orphan Receptor 1 (ROR1) Antibody (FITC)

abx449284-100ug 100 ug
EUR 727.2

Receptor Tyrosine Kinase Like Orphan Receptor 2 (ROR2) Antibody (FITC)

20-abx338153
  • EUR 493.20
  • EUR 2214.00
  • EUR 718.80
  • EUR 218.40
  • EUR 360.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Neuron Derived Orphan Receptor 1 (NOR1) Polyclonal Antibody (Rat), FITC

4-PAC675Ra01-FITC
  • EUR 373.20
  • EUR 3427.20
  • EUR 964.80
  • EUR 471.60
  • EUR 242.40
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Rat Neuron Derived Orphan Receptor 1 (NOR1). This antibody is labeled with FITC.

Receptor Tyrosine Kinase Like Orphan Receptor 2 (ROR2) Antibody (PerCP)

abx445971-100ug 100 ug
EUR 693.6

Receptor Tyrosine Kinase Like Orphan Receptor 1 (ROR1) Antibody (PerCP)

abx449269-100ug 100 ug
EUR 727.2

Receptor Tyrosine Kinase Like Orphan Receptor 1 (ROR1) Antibody (PerCP)

abx449287-100ug 100 ug
EUR 727.2

Receptor Tyrosine Kinase Like Orphan Receptor 1 (ROR1) Antibody (Biotin)

20-abx106039
  • EUR 493.20
  • EUR 2214.00
  • EUR 718.80
  • EUR 218.40
  • EUR 360.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Receptor Tyrosine Kinase Like Orphan Receptor 2 (ROR2) Antibody (Biotin)

abx445967-100ug 100 ug
EUR 693.6

Receptor Tyrosine Kinase Like Orphan Receptor 1 (ROR1) Antibody (Biotin)

abx449265-100ug 100 ug
EUR 727.2

Receptor Tyrosine Kinase Like Orphan Receptor 1 (ROR1) Antibody (Biotin)

abx449283-100ug 100 ug
EUR 727.2

Receptor Tyrosine Kinase Like Orphan Receptor 2 (ROR2) Antibody (Biotin)

20-abx338154
  • EUR 493.20
  • EUR 2214.00
  • EUR 718.80
  • EUR 218.40
  • EUR 360.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

RAR Related Orphan Receptor Alpha (RORa) Polyclonal Antibody (Human, Mouse)

4-PAD947Hu01
  • EUR 296.40
  • EUR 3012.00
  • EUR 750.00
  • EUR 372.00
  • EUR 256.80
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Human, Mouse RAR Related Orphan Receptor Alpha (RORa)

RAR Related Orphan Receptor Gamma (RORg) Polyclonal Antibody (Human, Mouse)

4-PAD949Hu01
  • EUR 296.40
  • EUR 3012.00
  • EUR 750.00
  • EUR 372.00
  • EUR 256.80
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Human, Mouse RAR Related Orphan Receptor Gamma (RORg)

Receptor Tyrosine Kinase Like Orphan Receptor 2 (ROR2) Antibody (ATTO 390)

abx445957-100ug 100 ug
EUR 693.6

Optimized broad-range real-time PCR-based method for bacterial screening of platelet concentrates

Bacterial contamination of blood components remains a major challenge in transfusion medicine, particularly, platelet concentrates (PCs) due to the storage conditions that support bacterial proliferation. In this study, we develop a rapid, sensitive and specific real-time PCR protocol for bacterial screening of PCs. An internally controlled real-time PCR-based method was optimized and validated with our proprietary 16S Universal PCR Master Mix (IBMP/Fiocruz), which targets a conserved region of the bacterial 16S rRNA gene. Nonspecific background DNA was completely eliminated by treating the PCR Master Mix with ethidium monoazide (EMA).

A lower limit of detection was observed for 10 genome equivalents with an observed Ct value of 34±1.07 in calibration curve generated with 10-fold serial dilutions of E. coli DNA. The turnaround time for processing, including microbial DNA purification, was approximately 4 hours. The developed method showed a high sensitivity with no non-specific amplification and a lower time-to-detection than traditional microbiological methods, demonstrating it to be an efficient means of screening pre-transfusion PCs.

Evaluation of sample pooling for diagnosis of COVID-19 by Real time PCR– A resource saving combat strategy

Background& objectives: Although about 80% of COVID-19 cases are reported to be mild, the remaining 20% of cases often result in a severe disease with the potential of crushing already overstrained health care services. There has been sustainable growth of COVID-19 cases worldwide since mid-May 2020. In order to keep tabs on community transmission of COVID-19 infection screening of the samples from large population is needed which includes asymptomatic/symptomatic individuals along with migrant population. This requires extra resources, man power and time for detection of SARS-CoV-2by RT-PCR. In current scenario pooled sample testingstrategy advocated by ICMR, New Delhiis a new approach which is very promising in resource limited settings. In this study we have evaluated pooled strategy in terms of accurate testing results, utilization of consumables and identification of border line positive cases.

Materials & methods: Between April-June 2020 we performed COVID-19 testing by RTPCR from areas with varying prevalence of population referred to COVID laboratory, Dr RMLIMS, Lucknow. In a first step the samples are collated into pools of 5 or 10.These pools are tested by RT-PCR. Negative Pools were reported as negative whereas positive pools of 5 and 10 are then deconvoluted and each sample is tested individually.

Results: In the present study, we tested 4620 samples in 462 pools of 10 and 14940 samples in 2990 pools of 5. Among 10 samples pool 61 (13%) pools flagged positive in first step. In second step among 61 pools (610 samples) deconvoluted strategy was followed in which 72 individual samples came positive. Pooled-sample testing strategy helps saves substantial resources and time during surge testing and enhanced pandemic surveillance. This approach requires around 76% to 93% less tests done in low to moderate prevalence settings and group sizes up to 5-10 in a population, compared to individual testing.

Conclusions: Pooled-sample PCR analysis strategies can save substantial resources and time for COVID-19 mass testing in comparison to individual testing without compromising the result outcome of test. In particular, pooled-sample approach can facilitate mass screening in early coming stages of COVID-19 outbreaks, especially in low- and middle-income settings and control the spread by meticulous testing of all risk groups. This article is protected by copyright. All rights reserved.

PCR SuperMix

20-abx098887
  • EUR 326.40
  • EUR 693.60
  • 1 ml
  • 5 ml

PCR Enhancer

P021-01 500 ul
EUR 132

PCR Stimulant

abx098001-200ul 200 ul
EUR 210

High Fidelity (HiFi) PCR SuperMix (-dye) (DNA, PCR)

20-abx098007
  • EUR 260.40
  • EUR 427.20
  • 1 ml
  • 5 ml

Eppendorf Mixmate PCR Tube Holder PCR For Tubes Strips Or 96 Well PCR Plates

E5353040113 EACH
EUR 114

Adhesive PCR Plate Clear Films for PCR Applications

GP502 100 sheets
EUR 306

PCR Tubes (50)

PCR-50 50
EUR 15
Description: To be used with the PractiChrom kits in conjuction with the PiCO Explorer. Quantity: 50 PCR tubes.

Leaf PCR Kit

11140007-1 1 Kit
EUR 121.1

Blood PCR Kit

20-abx09801320ulSystems
  • EUR 309.60
  • EUR 678.00
  • 100 rxns × 20 ul Systems
  • 500 rxns × 20 ul Systems

Ready? PCR Mix

M1127-1000 each
EUR 614.4

Ready? PCR Mix

M1127-200 each
EUR 229.2

Rigor? PCR Mix

M1132-200 each
EUR 385.2

Breeze? PCR Mix

M1134-200 each
EUR 320.4

PCR Buffer Set

11170001-1 1.25 mL
EUR 26.39
Description: PCR Buffer and MgCl2 Solution

Distant? PCR Mix

M1136-200 each
EUR 352.8

Advance? PCR Mix

M1139-200 each
EUR 451.2

PCR GRADE WATER

IB42300 1 VIAL
EUR 7.4
Description: (1.8ML IN 2.0ML VIAL)

PCR GRADE WATER

IB42301 20/PACK
EUR 128.43
Description: (1.8ML IN 2.0ML VIALS)

PCR GRADE WATER

IB42302 50/PACK
EUR 318.01
Description: (1.8ML IN 2.0ML VIALS)

PCR GRADE WATER

IB42303 100/PACK
EUR 633.73
Description: (1.8ML IN 2.0ML VIALS)

Water, PCR Grade

42300013-1 100 mL
EUR 21.5

Water, PCR Grade

42300013-2 250 mL
EUR 34.83

Water, PCR Grade

42300013-3 500 mL
EUR 60.1

Water, PCR Grade

42300013-4 1 L
EUR 82.47

PCR SuperMix (+dye)

20-abx098003
  • EUR 493.20
  • EUR 226.80
  • EUR 7408.80
  • EUR 309.60
  • 15 ml
  • 1 ml
  • 480 ml
  • 5 ml

PCR SuperMix (-dye)

20-abx098002
  • EUR 493.20
  • EUR 226.80
  • EUR 309.60
  • 15 ml
  • 1 ml
  • 5 ml

Plus PCR SuperMix

20-abx098888
  • EUR 292.80
  • EUR 560.40
  • 1 ml
  • 5 ml

Adhesive PCR Film

AB-0558 PK100
EUR 201.6

Adhesive PCR Foil

AB-0626 PK100
EUR 214.8

Multiplex PCR Kit

PM101-01 50 rxn
EUR 244.8

Multiplex PCR Kit

PM101-02 200 rxn
EUR 564

Multiplex PCR Kit

PM101-03 1000 rxn
EUR 2006.4

Sapovirus PCR kit

PCR-H630-48R 50T
EUR 987.6

Sapovirus PCR kit

PCR-H630-96R 100T
EUR 1335.6

Entamoeba PCR kit

PCR-H683-48D 50T
EUR 543.6

Entamoeba PCR kit

PCR-H683-96D 100T
EUR 686.4

Echovirus PCR kit

PCR-H722-48D 50T
EUR 543.6

Echovirus PCR kit

PCR-H722-96D 100T
EUR 686.4

ranavirus PCR kit

PCR-V337-48D 50T
EUR 543.6

ranavirus PCR kit

PCR-V337-96D 100T
EUR 686.4

PCR-grade Water

PCR-258-100 100ml
EUR 32.7

PCR-grade Water

PCR-258-1L 1L
EUR 283.6

PCR-grade Water

PCR-258-500 500ml
EUR 45.8

PCR-grade Water

PCR-258L 50ml
EUR 23.3

PCR-grade Water

PCR-258S 10 x 1,2ml
EUR 20

Parvovirus В19 PCR kit

PCR-H455-48D 50T
EUR 543.6

Parvovirus В19 PCR kit

PCR-H455-96D 100T
EUR 686.4

MM-Separator 384 PCR

MDMG0006 1
EUR 1914

Double PCR Cabinet

CAB1003 EACH
EUR 5028

Mengovirus PCR kit

PCR-H453-48R 50T
EUR 987.6

Mengovirus PCR kit

PCR-H453-96R 100T
EUR 1335.6

Astrovirus PCR kit

PCR-H491-48R 50T
EUR 987.6

Astrovirus PCR kit

PCR-H491-96R 100T
EUR 1335.6

Adenovirus PCR kit

PCR-H501-48D 50T
EUR 543.6

Adenovirus PCR kit

PCR-H501-96D 100T
EUR 686.4

amfiEco PCR Premix

P0702-096 96 rxns
EUR 186

amfiEco PCR Premix

P0702-480 5x96 rxns
EUR 532.8

amfiEco PCR Premix

P0702-960 10x96 rxns
EUR 984

PCR-TOPO-KCNKP

PVT17547 2 ug
EUR 277.2

Economy PCR Cabinet

CAB1001 EACH
EUR 2904

Enterovirus PCR kit

PCR-H433-48R 50T
EUR 987.6

Enterovirus PCR kit

PCR-H433-96R 100T
EUR 1030.8

Calicivirus PCR kit

PCR-H493-48R 50T
EUR 987.6

Calicivirus PCR kit

PCR-H493-96R 100T
EUR 1335.6

Water, PCR Certified

W0806-010 100ml
EUR 105.6

Water, PCR Certified

W0806-050 500ml
EUR 134.4

Water, PCR Certified

W0806-100 10x10ml
EUR 116.4

PCR Purification Kit

20-abx098084
  • EUR 326.40
  • EUR 243.60
  • 200 rxns
  • 50 rxns

Ruminococcus PCR kit

PCR-V619-48D 50T
EUR 510.96
Description: An conventional PCR kit for detection of Ruminococcus

Ruminococcus PCR kit

PCR-V619-96D 100T
EUR 625.8
Description: An conventional PCR kit for detection of Ruminococcus

Halobacillus PCR kit

PCR-A635-48D 50T
EUR 543.6

Halobacillus PCR kit

PCR-A635-96D 100T
EUR 686.4

Polyomavirus PCR kit

PCR-H515-48D 50T
EUR 543.6

Polyomavirus PCR kit

PCR-H515-96D 100T
EUR 686.4

Ruminococcus PCR kit

PCR-H619-48D 48T
EUR 543.6

Ruminococcus PCR kit

PCR-H619-96D 96T
EUR 686.4

Parechovirus PCR kit

PCR-H642-48R 50T
EUR 813.6

Parechovirus PCR kit

PCR-H642-96R 100T
EUR 1335.6

Acanthamoeba PCR kit

PCR-H684-48D 50T
EUR 543.6

Acanthamoeba PCR kit

PCR-H684-96D 100T
EUR 686.4

Multiplex PCR Master

PCR-110L 10 x 1,25ml
EUR 891.2

Multiplex PCR Master

PCR-110S 2 x 1,25ml
EUR 222.8

bioPLUS PCR Enhancer

11170003-1 5 mL
EUR 48.49

PCR magnetic Cleanup

TBS6029-200 200 Tests
EUR 189

Methylamp PCR Enhancer 

R-1002
  • EUR 185.28
  • EUR 90.20
  • 400 Reactions
  • 400 Reactions