A Simplified Quantitative Real-Time PCR Assay for Monitoring SARS-CoV-2 Growth in Cell Culture

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has infected millions within just a few months, causing severe respiratory disease and mortality. Assays to monitor SARS-CoV-2 growth in vitro depend on time-consuming and costly RNA extraction steps, hampering progress in basic research and drug development efforts. Here, we developed a simplified quantitative real-time PCR assay that bypasses viral RNA extraction steps and can monitor SARS-CoV-2 growth from a small amount of cell culture supernatants. In addition, we show that this approach is easily adaptable to numerous other RNA and DNA viruses. Using this assay, we screened the activities of a number of compounds that were predicted to alter SARS-CoV-2 entry and replication as well as HIV-1-specific drugs in a proof-of-concept study. We found that E64D (inhibitor of endosomal proteases cathepsin B and L) and apilimod (endosomal trafficking inhibitor) potently decreased the amount of SARS-CoV-2 RNA in cell culture supernatants with minimal cytotoxicity.

Surprisingly, we found that the macropinocytosis inhibitor ethylisopropylamiloride (EIPA) similarly decreased SARS-CoV-2 RNA levels in supernatants, suggesting that entry may additionally be mediated by an alternative pathway. HIV-1-specific inhibitors nevirapine (a nonnucleoside reverse transcriptase inhibitor [NNRTI]), amprenavir (a protease inhibitor), and allosteric integrase inhibitor 2 (ALLINI-2) modestly inhibited SARS-CoV-2 replication, albeit the 50% inhibitory concentration (IC₅₀) values were much higher than that required for HIV-1.

Taking the data together, this simplified assay will expedite basic SARS-CoV-2 research, be amenable to mid-throughput screening assays (i.e., drug, CRISPR, small interfering RNA [siRNA], etc.), and be applicable to a broad number of RNA and DNA viruses.IMPORTANCE Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the etiological agent of the coronavirus disease 2019 (COVID-19) pandemic, is continuing to cause immense respiratory disease and social and economic disruptions. Conventional assays that monitor SARS-CoV-2 growth in cell culture rely on costly and time-consuming RNA extraction procedures, hampering progress in basic SARS-CoV-2 research and development of effective therapeutics. Here, we developed a simple quantitative real-time PCR assay to monitor SARS-CoV-2 growth in cell culture supernatants that does not necessitate RNA extraction and that is as accurate and sensitive as existing methods. In a proof-of-concept screen, we found that E64D, apilimod, EIPA, and remdesivir can substantially impede SARS-Cov-2 replication, providing novel insight into viral entry and replication mechanisms. In addition, we show that this approach is easily adaptable to numerous other RNA and DNA viruses. This simplified assay will undoubtedly expedite basic SARS-CoV-2 and virology research and be amenable to use in drug screening platforms to identify therapeutics against SARS-CoV-2.

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Nori® Equine 6-Plex ELISA Kits-TLR
GR225028	Genorise Scientific	96-well	EUR 629

Nori® Equine 6-Plex ELISA Kits-SAA
GR225030	Genorise Scientific	96-well	EUR 629

Nori® Canine 6-Plex ELISA Kits-BMP
GR226025	Genorise Scientific	96-well	EUR 629

Nori® Canine 6-Plex ELISA Kits-CCL
GR226026	Genorise Scientific	96-well	EUR 629

Nori® Canine 6-Plex ELISA Kits-MMP
GR226027	Genorise Scientific	96-well	EUR 629

Nori® Canine 6-Plex ELISA Kits-TLR
GR226028	Genorise Scientific	96-well	EUR 629

Nori® Canine 6-Plex ELISA Kits-SAA
GR226030	Genorise Scientific	96-well	EUR 629

Nori® Bovine 6-Plex ELISA Kits-BMP
GR228025	Genorise Scientific	96-well	EUR 629

Nori® Bovine 6-Plex ELISA Kits-CCL
GR228026	Genorise Scientific	96-well	EUR 629

Nori® Bovine 6-Plex ELISA Kits-MMP
GR228027	Genorise Scientific	96-well	EUR 629

Nori® Bovine 6-Plex ELISA Kits-TLR
GR228028	Genorise Scientific	96-well	EUR 629

Nori® Bovine 6-Plex ELISA Kits-SAA
GR228030	Genorise Scientific	96-well	EUR 629

Nori® Porcine 6-Plex ELISA Kits-BMP
GR224025	Genorise Scientific	96-well	EUR 629

Nori® Porcine 6-Plex ELISA Kits-CCL
GR224026	Genorise Scientific	96-well	EUR 629

Nori® Porcine 6-Plex ELISA Kits-MMP
GR224027	Genorise Scientific	96-well	EUR 629

Nori® Porcine 6-Plex ELISA Kits-TLR
GR224028	Genorise Scientific	96-well	EUR 629

Nori® Porcine 6-Plex ELISA Kits-SAA
GR224030	Genorise Scientific	96-well	EUR 629

Nori® Equine 6-Plex ELISA Kits-S100
GR225031	Genorise Scientific	96-well	EUR 629

Nori® Equine 6-Plex ELISA Kits-CXCL
GR225032	Genorise Scientific	96-well	EUR 629

Nori® Canine 6-Plex ELISA Kits-S100
GR226031	Genorise Scientific	96-well	EUR 629

Nori® Canine 6-Plex ELISA Kits-CXCL
GR226032	Genorise Scientific	96-well	EUR 629

Nori® Bovine 6-Plex ELISA Kits-S100
GR228031	Genorise Scientific	96-well	EUR 629

Nori® Bovine 6-Plex ELISA Kits-CXCL
GR228032	Genorise Scientific	96-well	EUR 629

Nori® Porcine 6-Plex ELISA Kits-S100
GR224031	Genorise Scientific	96-well	EUR 629

Nori® Porcine 6-Plex ELISA Kits-CXCL
GR224032	Genorise Scientific	96-well	EUR 629

Nori® Equine 6-Plex ELISA Kits-Hormones
GR225029	Genorise Scientific	96-well	EUR 629

Nori® Canine 6-Plex ELISA Kits-Hormones
GR226029	Genorise Scientific	96-well	EUR 629

Nori® Bovine 6-Plex ELISA Kits-Hormones
GR228029	Genorise Scientific	96-well	EUR 629

Nori® Porcine 6-Plex ELISA Kits-Hormones
GR224029	Genorise Scientific	96-well	EUR 629

Nori® Equine 3-Plex ELISA Kits-Interferons
GR225009	Genorise Scientific	96-well	EUR 503

Nori® Canine 3-Plex ELISA Kits-Interferons
GR226009	Genorise Scientific	96-well	EUR 503

Nori® Bovine 3-Plex ELISA Kits-Interferons
GR228009	Genorise Scientific	96-well	EUR 503

Nori® Porcine 3-Plex ELISA Kits-Interferons
GR224009	Genorise Scientific	96-well	EUR 503

EdU Imaging Kits (488)
K1175-50	ApexBio	50-500 tests	EUR 240
Description: Cell Biology|Cell Proliferation & Toxicity

BSA Standard kits
TBS5002	Tribioscience	7x 1ml	EUR 45

Nori® Equine 4-Plex ELISA Kits-Immunoglobulins
GR225017	Genorise Scientific	96-well	EUR 545

Nori® Canine 4-Plex ELISA Kits-Immunoglobulins
GR226017	Genorise Scientific	96-well	EUR 545

Nori® Bovine 4-Plex ELISA Kits-Immunoglobulins
GR228017	Genorise Scientific	96-well	EUR 545

Tricorn Filter Kits
TFK-013C	Creative BioMart	each	EUR 320
Description: Chromatography Reagents

Nori® Porcine 4-Plex ELISA Kits-Immunoglobulins
GR224017	Genorise Scientific	96-well	EUR 545

Human Glutaminase Liver Isoform, Mitochondrial (GLS2) ELISA Kits
EIA07209h	Enlibio	each	EUR 430

Nori® Equine 6-Plex ELISA Kits-Cardiac Panel
GR225034	Genorise Scientific	96-well	EUR 629

Nori® Canine 6-Plex ELISA Kits-Cardiac Panel
GR226034	Genorise Scientific	96-well	EUR 629

Nori® Bovine 6-Plex ELISA Kits-Cardiac Panel
GR228034	Genorise Scientific	96-well	EUR 629

Bovine Glutaminase Liver Isoform, Mitochondrial (GLS2) ELISA Kits
MBS2614905-10x96StripWells	MyBiosource	10x96-Strip-Wells	EUR 3545

Bovine Glutaminase Liver Isoform, Mitochondrial (GLS2) ELISA Kits
MBS2614905-48StripWells	MyBiosource	48-Strip-Wells	EUR 310

Bovine Glutaminase Liver Isoform, Mitochondrial (GLS2) ELISA Kits
MBS2614905-5x96StripWells	MyBiosource	5x96-Strip-Wells	EUR 1940

Bovine Glutaminase Liver Isoform, Mitochondrial (GLS2) ELISA Kits
MBS2614905-96StripWells	MyBiosource	96-Strip-Wells	EUR 475

Nori® Porcine 6-Plex ELISA Kits-Cardiac Panel
GR224034	Genorise Scientific	96-well	EUR 629

Nori® Equine 6-Plex ELISA Kits-Growth Factors
GR225024	Genorise Scientific	96-well	EUR 629

Nori® Canine 6-Plex ELISA Kits-Growth Factors
GR226024	Genorise Scientific	96-well	EUR 629

Nori® Bovine 6-Plex ELISA Kits-Growth Factors
GR228024	Genorise Scientific	96-well	EUR 629

Nori® Porcine 6-Plex ELISA Kits-Growth Factors
GR224024	Genorise Scientific	96-well	EUR 629

Nori® Equine 6-Plex ELISA Kits-Inflammation Panel
GR225033	Genorise Scientific	96-well	EUR 629

Nori® Canine 6-Plex ELISA Kits-Inflammation Panel
GR226033	Genorise Scientific	96-well	EUR 629

Nori® Bovine 6-Plex ELISA Kits-Inflammation Panel
GR228033	Genorise Scientific	96-well	EUR 629

Nori® Porcine 6-Plex ELISA Kits-Inflammation Panel
GR224033	Genorise Scientific	96-well	EUR 629

T&A Cloning Kits
FYC001-20P	Yeastern Biotech	1 vial	Ask for price

EdU Imaging Kits (Cy3)
K1075-50	ApexBio	50-500 tests	EUR 240
Description: EdU

EdU Imaging Kits (Cy5)
K1076-50	ApexBio	50-500 tests	EUR 240
Description: EdU

Nori® Equine 3-Plex ELISA Kits-IL6-IL8-TNFA
GR225005	Genorise Scientific	96-well	EUR 503

Nori® Equine 3-Plex ELISA Kits-IL1B-IL2-IL4
GR225008	Genorise Scientific	96-well	EUR 503

Nori® Canine 3-Plex ELISA Kits-IL6-IL8-TNFA
GR226005	Genorise Scientific	96-well	EUR 503

Nori® Canine 3-Plex ELISA Kits-IL1B-IL2-IL4
GR226008	Genorise Scientific	96-well	EUR 503

Nori® Bovine 3-Plex ELISA Kits-IL6-IL8-TNFA
GR228005	Genorise Scientific	96-well	EUR 503

Nori® Bovine 3-Plex ELISA Kits-IL1B-IL2-IL4
GR228008	Genorise Scientific	96-well	EUR 503

Nori® Porcine 3-Plex ELISA Kits-IL6-IL8-TNFA
GR224005	Genorise Scientific	96-well	EUR 503

Nori® Porcine 3-Plex ELISA Kits-IL1B-IL2-IL4
GR224008	Genorise Scientific	96-well	EUR 503

Nori® Equine 3-Plex ELISA Kits-IL1A-IL8-TNFA
GR225001	Genorise Scientific	96-well	EUR 503

Nori® Equine 3-Plex ELISA Kits-IL1B-IL8-TNFA
GR225002	Genorise Scientific	96-well	EUR 503

Nori® Equine 3-Plex ELISA Kits-IL1B-IL6-TNFA
GR225003	Genorise Scientific	96-well	EUR 503

Nori® Equine 3-Plex ELISA Kits-IL6-IL10-TNFA
GR225006	Genorise Scientific	96-well	EUR 503

Nori® Canine 3-Plex ELISA Kits-IL1A-IL8-TNFA
GR226001	Genorise Scientific	96-well	EUR 503

Nori® Canine 3-Plex ELISA Kits-IL1B-IL8-TNFA
GR226002	Genorise Scientific	96-well	EUR 503

Nori® Canine 3-Plex ELISA Kits-IL1B-IL6-TNFA
GR226003	Genorise Scientific	96-well	EUR 503

Nori® Canine 3-Plex ELISA Kits-IL6-IL10-TNFA
GR226006	Genorise Scientific	96-well	EUR 503

Nori® Bovine 3-Plex ELISA Kits-IL1A-IL8-TNFA
GR228001	Genorise Scientific	96-well	EUR 503

Nori® Bovine 3-Plex ELISA Kits-IL1B-IL8-TNFA
GR228002	Genorise Scientific	96-well	EUR 503

Nori® Bovine 3-Plex ELISA Kits-IL1B-IL6-TNFA
GR228003	Genorise Scientific	96-well	EUR 503

Nori® Bovine 3-Plex ELISA Kits-IL6-IL10-TNFA
GR228006	Genorise Scientific	96-well	EUR 503

EdU Imaging Kits (HF488)
K2240-50	ApexBio	50-500tests	EUR 240
Description: Cell Biology|Cell Proliferation & Toxicity

EdU Imaging Kits (HF594)
K2243-50	ApexBio	50-500tests	EUR 240
Description: Cell Biology|Cell Proliferation & Toxicity

Nori® Porcine 3-Plex ELISA Kits-IL1A-IL8-TNFA
GR224001	Genorise Scientific	96-well	EUR 503

Nori® Porcine 3-Plex ELISA Kits-IL1B-IL8-TNFA
GR224002	Genorise Scientific	96-well	EUR 503

Nori® Porcine 3-Plex ELISA Kits-IL1B-IL6-TNFA
GR224003	Genorise Scientific	96-well	EUR 503

Nori® Porcine 3-Plex ELISA Kits-IL6-IL10-TNFA
GR224006	Genorise Scientific	96-well	EUR 503

Nori® Equine 3-Plex ELISA Kits-IL1B-IL10-TNFA
GR225004	Genorise Scientific	96-well	EUR 503

Nori® Canine 3-Plex ELISA Kits-IL1B-IL10-TNFA
GR226004	Genorise Scientific	96-well	EUR 503

Nori® Bovine 3-Plex ELISA Kits-IL1B-IL10-TNFA
GR228004	Genorise Scientific	96-well	EUR 503

Nori® Porcine 3-Plex ELISA Kits-IL1B-IL10-TNFA
GR224004	Genorise Scientific	96-well	EUR 503

Necrosis vs Apoptosis Kits
KF17371	Neuromics	50-100 Tests	EUR 522

Necrosis vs Apoptosis Kits
KF17372	Neuromics	100-200 Tests	EUR 921.6

Basic Cytotoxicity Test Kits
CT17001	Neuromics	125 Tests	EUR 490.8

Basic Cytotoxicity Test Kits
CT17002	Neuromics	250 Tests	EUR 780

Total Cytotoxicity Test Kits
CT17003	Neuromics	125 Tests	EUR 615.6

Total Cytotoxicity Test Kits
CT17004	Neuromics	250 Tests	EUR 975.6

Gel Filtration Calibration Kits
Ge-474C	Creative BioMart	1 Kit	EUR 1704
Description: Chromatography Reagents

Uncoated Ancillary Reagent Kits
RK04587	Abclonal	20Plates	EUR 294.03

Nori® Equine 3-Plex ELISA Kits-IL4-IL6-IL-10
GR225007	Genorise Scientific	96-well	EUR 503

Nori® Canine 3-Plex ELISA Kits-IL4-IL6-IL-10
GR226007	Genorise Scientific	96-well	EUR 503

Nori® Bovine 3-Plex ELISA Kits-IL4-IL6-IL-10
GR228007	Genorise Scientific	96-well	EUR 503

Nori® Porcine 3-Plex ELISA Kits-IL4-IL6-IL-10
GR224007	Genorise Scientific	96-well	EUR 503

Nori® Equine 6-Plex ELISA Kits-Type I Cytokine Panel
GR225035	Genorise Scientific	96-well	EUR 629

Nori® Equine 6-Plex ELISA Kits-Type II Cytokine Panel
GR225036	Genorise Scientific	96-well	EUR 629

Nori® Canine 6-Plex ELISA Kits-Type I Cytokine Panel
GR226035	Genorise Scientific	96-well	EUR 629

Nori® Canine 6-Plex ELISA Kits-Type II Cytokine Panel
GR226036	Genorise Scientific	96-well	EUR 629

Nori® Bovine 6-Plex ELISA Kits-Type I Cytokine Panel
GR228035	Genorise Scientific	96-well	EUR 629

Nori® Bovine 6-Plex ELISA Kits-Type II Cytokine Panel
GR228036	Genorise Scientific	96-well	EUR 629

Nori® Porcine 6-Plex ELISA Kits-Type I Cytokine Panel
GR224035	Genorise Scientific	96-well	EUR 629

Nori® Porcine 6-Plex ELISA Kits-Type II Cytokine Panel
GR224036	Genorise Scientific	96-well	EUR 629

Comparison of Droplet Digital PCR versus qPCR Measurements on the International Scale for the Molecular Monitoring of Chronic Myeloid Leukemia Patients

Background: BCR-ABL1/ABL1 p210 measurement by quantitative polymerase chain reaction (qPCR) is used worldwide to monitor the molecular response in chronic myeloid leukemia (CML) patients. Droplet digital polymerase chain reaction (ddPCR) seems to show a greater sensitivity than qPCR, probably due to the high number of replicates analyzed in ddPCR for the comparison. Additionally, in a recently published comparison, ddPCR measurements were not adequately transformed into International Scale (IS).

Method: We have analyzed 50 CML patients and ten non-CML donors in parallel by qPCR and ddPCR. To the best of our knowledge, this is the first study comparing both techniques under similar conditions, with BCR-ABL1/ABL1 measurements performed via both techniques transformed into IS.

Results: Qualitative and quantitative comparisons showed excellent results. The qualitative correlation showed a Kappa index of 0.94 (95% confidence interval [CI] 0.90-0.98) (P < 0.001). In the quantitative comparison, the absolute intra-class correlation coefficient was 0.868 (95% CI 0.734-0.937; P < 0.001), and Lin’s concordance correlation coefficient was 0.863. The Passing-Bablock test indicated a slight proportional difference between qPCR and ddPCR. A quantitative and qualitative subanalysis including 40 patients with a molecular response of 3.0 or deeper showed similar results in every test. In addition, the proportional difference in the Passing-Bablock test disappeared. There were no differences in the sensitivity for BCR-ABL1 detection between qPCR and ddPCR (McNemar test, P = 0.5).

Conclusions: In conclusion, our results show very good quantitative and qualitative correlations between BCR-ABL1/ABL1 p210 results obtained by qPCR and by ddPCR and confirm previous scarce data regarding the lack of an increase in sensitivity of ddPCR over qPCR in this setting.

OVA Conjugated Phosphocreatine (PCr)
MBS2097502-01mg	MyBiosource	0.1mg	EUR 375

OVA Conjugated Phosphocreatine (PCr)
MBS2097502-02mg	MyBiosource	0.2mg	EUR 460

OVA Conjugated Phosphocreatine (PCr)
MBS2097502-05mg	MyBiosource	0.5mg	EUR 860

OVA Conjugated Phosphocreatine (PCr)
RPU55131-100ug	Biomatik Corporation	100ug	EUR 431.2

OVA Conjugated Phosphocreatine (PCr)
RPU55131-1mg	Biomatik Corporation	1mg	EUR 1911

OVA Conjugated Phosphocreatine (PCr)
RPU55131-50ug	Biomatik Corporation	50ug	EUR 336

Phosphocreatine (PCr) (OVA)
abx651920-100g	Abbexa	100 µg	EUR 1800

Phosphocreatine (PCr) (OVA)
abx651920-10g	Abbexa	10 µg	EUR 475

Phosphocreatine (PCr) (OVA)
abx651920-50g	Abbexa	50 µg	EUR 575

Phosphocreatine (PCr) Protein (OVA)
20-abx651920	Abbexa	Ask for price Ask for price Ask for price Ask for price Ask for price	10 ug 50 ug 100 ug 200 ug 1 mg

Phosphocreatine (PCr) Antibody
abx132173-100tests	Abbexa	100 tests	EUR 287.5

Phosphocreatine (PCr) Antibody
20-abx132173	Abbexa	Ask for price Ask for price Ask for price Ask for price Ask for price	10 ug 50 ug 100 ug 200 ug 1 mg

Phosphocreatine (PCr) CLIA Kit
20-abx490605	Abbexa	Ask for price Ask for price Ask for price	96 tests 5 × 96 tests 10 × 96 tests

Phosphocreatine (PCr) CLIA Kit
EKU12351-48T	Biomatik Corporation	48T	EUR 801.78

Phosphocreatine (PCr) CLIA Kit
EKU12351-5x96T	Biomatik Corporation	5x96T	EUR 5440.65

Phosphocreatine (PCr) CLIA Kit
EKU12351-96T	Biomatik Corporation	96T	EUR 1145.4

Phosphocreatine (PCr) CLIA Kit
EKN53463-48T	Biomatik Corporation	48T	EUR 543.83

Phosphocreatine (PCr) CLIA Kit
EKN53463-5x96T	Biomatik Corporation	5x96T	EUR 3690.28

Phosphocreatine (PCr) CLIA Kit
EKN53463-96T	Biomatik Corporation	96T	EUR 776.9

Phosphocreatine (PCr) ELISA Kit
20-abx258965	Abbexa	Ask for price Ask for price Ask for price	96 tests 5 × 96 tests 10 × 96 tests

Phosphocreatine (PCr) ELISA Kit
abx258965-1mg	Abbexa	1 mg	EUR 6525

Phosphocreatine (PCr) ELISA Kit
abx258965-20g	Abbexa	20 µg	EUR 3450

Phosphocreatine (PCr) ELISA Kit
abx258965-5g	Abbexa	5 µg	EUR 725

Phosphocreatine (PCr) ELISA Kit
EKU11336-48T	Biomatik Corporation	48T	EUR 659.26

Phosphocreatine (PCr) ELISA Kit
EKU11336-5x96T	Biomatik Corporation	5x96T	EUR 4473.55

Phosphocreatine (PCr) ELISA Kit
EKU11336-96T	Biomatik Corporation	96T	EUR 941.8

Phosphocreatine (PCr) ELISA Kit
EKN52447-48T	Biomatik Corporation	48T	EUR 409.92

Phosphocreatine (PCr) ELISA Kit
EKN52447-5x96T	Biomatik Corporation	5x96T	EUR 2781.6

Phosphocreatine (PCr) ELISA Kit
EKN52447-96T	Biomatik Corporation	96T	EUR 585.6

PCr(Phosphocreatine)ELISAKit
ELK8254-48T	ELK Biotech	48T	EUR 133
Description: Competitive Inhibition

PCr(Phosphocreatine)ELISAKit
ELK8254-96T	ELK Biotech	96T	EUR 188
Description: Competitive Inhibition

CLIA Kit for Phosphocreatine (PCr)
CCV808Ge	Cloud-Clone	96Т	EUR 996

Rat Phosphocreatine(Pcr) ELISA Kit
CK-bio-25842-48T	Shanghai Coon Koon Biotech	48T	Ask for price

Rat Phosphocreatine(Pcr) ELISA Kit
CK-bio-25842-96T	Shanghai Coon Koon Biotech	96T	Ask for price

ELISA Kit for Phosphocreatine (PCr)
CEV808Ge	Cloud-Clone	96Т	EUR 830

Human Phosphocreatine (PCr) ELISA Kit
SL2901Hu	Sunlong	96 Tests	EUR 468

Phosphocreatine (PCr) Polyclonal Antibody
CAU30010-100ul	Biomatik Corporation	100ul	EUR 267.8

Phosphocreatine (PCr) Polyclonal Antibody
CAU30010-200ul	Biomatik Corporation	200ul	EUR 334.3

General Phosphocreatine (PCr) CLIA Kit
MBS2127152-10x96StripWells	MyBiosource	10x96-Strip-Wells	EUR 7155

General Phosphocreatine (PCr) CLIA Kit
MBS2127152-24StripWells	MyBiosource	24-Strip-Wells	EUR 460

General Phosphocreatine (PCr) CLIA Kit
MBS2127152-48StripWells	MyBiosource	48-Strip-Wells	EUR 720

General Phosphocreatine (PCr) CLIA Kit
MBS2127152-5x96StripWells	MyBiosource	5x96-Strip-Wells	EUR 3940

General Phosphocreatine (PCr) CLIA Kit
MBS2127152-96StripWells	MyBiosource	96-Strip-Wells	EUR 980

General Phosphocreatine (PCr) ELISA Kit
CEV808Ge-10x96wellstestplate	Cloud-Clone	10x96-wells test plate	EUR 6698.82
Description: This is Competitive Enzyme-linked immunosorbent assay for detection of General Phosphocreatine (PCr) in serum, plasma and other biological fluids.

General Phosphocreatine (PCr) ELISA Kit
CEV808Ge-1x48wellstestplate	Cloud-Clone	1x48-wells test plate	EUR 656.84
Description: This is Competitive Enzyme-linked immunosorbent assay for detection of General Phosphocreatine (PCr) in serum, plasma and other biological fluids.

General Phosphocreatine (PCr) ELISA Kit
CEV808Ge-1x96wellstestplate	Cloud-Clone	1x96-wells test plate	EUR 886.92
Description: This is Competitive Enzyme-linked immunosorbent assay for detection of General Phosphocreatine (PCr) in serum, plasma and other biological fluids.

General Phosphocreatine (PCr) ELISA Kit
CEV808Ge-5x96wellstestplate	Cloud-Clone	5x96-wells test plate	EUR 3631.14
Description: This is Competitive Enzyme-linked immunosorbent assay for detection of General Phosphocreatine (PCr) in serum, plasma and other biological fluids.

General Phosphocreatine (PCr) ELISA Kit
4-CEV808Ge	Cloud-Clone	Ask for price Ask for price Ask for price	1 plate of 96 wells 5 plates of 96 wells 10 plates of 96 wells
Description: Enzyme-linked immunosorbent assay based on the Competitive Inhibition method for detection of General Phosphocreatine (PCr) in samples from serum, plasma and other biological fluids with no significant corss-reactivity with analogues from other species.

Chicken Phosphocreatine,Pcr ELISA KIT
JOT-EKA0067Ch	Jotbody	96 wells	EUR 398.4
Description: Chicken

Chicken Phosphocreatine,Pcr ELISA KIT
EA0067Ch	Jiaxing Korain Biotech Ltd (BT Labs)	96 wells	EUR 498

General Phosphocreatine (PCr) ELISA Kit
MBS2090465-10x96StripWells	MyBiosource	10x96-Strip-Wells	EUR 6145

General Phosphocreatine (PCr) ELISA Kit
MBS2090465-24StripWells	MyBiosource	24-Strip-Wells	EUR 395

General Phosphocreatine (PCr) ELISA Kit
MBS2090465-48StripWells	MyBiosource	48-Strip-Wells	EUR 620

General Phosphocreatine (PCr) ELISA Kit
MBS2090465-5x96StripWells	MyBiosource	5x96-Strip-Wells	EUR 3365

General Phosphocreatine (PCr) ELISA Kit
MBS2090465-96StripWells	MyBiosource	96-Strip-Wells	EUR 835

General Phosphocreatine (PCr) ELISA Kit
MBS2700694-10x96StripWells	MyBiosource	10x96-Strip-Wells	EUR 3690

General Phosphocreatine (PCr) ELISA Kit
MBS2700694-24StripWells	MyBiosource	24-Strip-Wells	EUR 270

General Phosphocreatine (PCr) ELISA Kit
MBS2700694-48StripWells	MyBiosource	48-Strip-Wells	EUR 365

General Phosphocreatine (PCr) ELISA Kit
MBS2700694-5x96StripWells	MyBiosource	5x96-Strip-Wells	EUR 1915

General Phosphocreatine (PCr) ELISA Kit
MBS2700694-96StripWells	MyBiosource	96-Strip-Wells	EUR 475

Polyclonal Antibody to Phosphocreatine (PCr)
MBS2090483-01mL	MyBiosource	0.1mL	EUR 190

Polyclonal Antibody to Phosphocreatine (PCr)
MBS2090483-02mL	MyBiosource	0.2mL	EUR 235

Polyclonal Antibody to Phosphocreatine (PCr)
MBS2090483-05mL	MyBiosource	0.5mL	EUR 400

Polyclonal Antibody to Phosphocreatine (PCr)
MBS2090483-1mL	MyBiosource	1mL	EUR 485

Polyclonal Antibody to Phosphocreatine (PCr)
MBS2090483-5mL	MyBiosource	5mL	EUR 1345

Polyclonal Antibody to Phosphocreatine (PCr)
PAV808Ge01	Cloud-Clone	100ul	EUR 279

General PCr (Phosphocreatine) ELISA Kit
MBS8807214-10x96StripWells	MyBiosource	10x96-Strip-Wells	EUR 3130

General PCr (Phosphocreatine) ELISA Kit
MBS8807214-48StripWells	MyBiosource	48-Strip-Wells	EUR 315

General PCr (Phosphocreatine) ELISA Kit
MBS8807214-5x96StripWells	MyBiosource	5x96-Strip-Wells	EUR 1710

General PCr (Phosphocreatine) ELISA Kit
MBS8807214-96StripWells	MyBiosource	96-Strip-Wells	EUR 385

General Phosphocreatine ELISA Kit (PCr)
RK00700	Abclonal	96 Tests	EUR 368.42

Phosphocreatine (PCr) Magnetic Luminex Assay Kit
LKU607319-96T	Biomatik Corporation	96T	EUR 1145.4

ELISA kit for General PCr (Phosphocreatine)
ELK8254	ELK Biotech	1 plate of 96 wells	EUR 446.4
Description: A competitive Inhibition ELISA kit for detection of Phosphocreatine from General in samples from blood, serum, plasma, cell culture fluid and other biological fluids.

Phosphocreatine (PCr) Polyclonal Antibody (Pan-species)
4-PAV808Ge01	Cloud-Clone	Ask for price Ask for price Ask for price Ask for price Ask for price	20ul 100ul 200ul 1ml 10ml
Description: A Rabbit polyclonal antibody against Pan-species Phosphocreatine (PCr)

PE-Linked Polyclonal Antibody to Phosphocreatine (PCr)
MBS2110977-01mL	MyBiosource	0.1mL	EUR 295

PE-Linked Polyclonal Antibody to Phosphocreatine (PCr)
MBS2110977-02mL	MyBiosource	0.2mL	EUR 400

PE-Linked Polyclonal Antibody to Phosphocreatine (PCr)
MBS2110977-05mL	MyBiosource	0.5mL	EUR 740

PE-Linked Polyclonal Antibody to Phosphocreatine (PCr)
MBS2110977-1mL	MyBiosource	1mL	EUR 915

PE-Linked Polyclonal Antibody to Phosphocreatine (PCr)
MBS2110977-5mL	MyBiosource	5mL	EUR 2640

APC-Linked Polyclonal Antibody to Phosphocreatine (PCr)
MBS2110978-01mL	MyBiosource	0.1mL	EUR 295

APC-Linked Polyclonal Antibody to Phosphocreatine (PCr)
MBS2110978-02mL	MyBiosource	0.2mL	EUR 400

APC-Linked Polyclonal Antibody to Phosphocreatine (PCr)
MBS2110978-05mL	MyBiosource	0.5mL	EUR 740

APC-Linked Polyclonal Antibody to Phosphocreatine (PCr)
MBS2110978-1mL	MyBiosource	1mL	EUR 915

APC-Linked Polyclonal Antibody to Phosphocreatine (PCr)
MBS2110978-5mL	MyBiosource	5mL	EUR 2640

Cy3-Linked Polyclonal Antibody to Phosphocreatine (PCr)
MBS2110979-01mL	MyBiosource	0.1mL	EUR 295

Cy3-Linked Polyclonal Antibody to Phosphocreatine (PCr)
MBS2110979-02mL	MyBiosource	0.2mL	EUR 400

Cy3-Linked Polyclonal Antibody to Phosphocreatine (PCr)
MBS2110979-05mL	MyBiosource	0.5mL	EUR 740

Cy3-Linked Polyclonal Antibody to Phosphocreatine (PCr)
MBS2110979-1mL	MyBiosource	1mL	EUR 915

Cy3-Linked Polyclonal Antibody to Phosphocreatine (PCr)
MBS2110979-5mL	MyBiosource	5mL	EUR 2640

HRP-Linked Polyclonal Antibody to Phosphocreatine (PCr)
MBS2110982-01mL	MyBiosource	0.1mL	EUR 220